I'd like to know what others have seen in various methods concerning mixed field samples. We are a children's hospital who transfuses a large amount of O red blood cells. We're seeing mixed field reactions when doing blood typing on approximately 25% of our patient samples! We use gel technology for our ab screens and DATs, but are still using tube method for ABO/Rh. As we look to the future of automation or possibly changing our ABO/Rh testing to gel technique, I'd like to hear what have others experienced concerning mixed field. I liked what I saw with the ECHO demos until I discovered that it cannot read mixed-field and would kick out up to a quarter of our ABO results! Any better luck with gel - manual or using the Provue? Stephanie