This gets me everytime! Now I am trying to write a procedure. According to the Technical Manual, 16th Edition, it states that RBCs collected in AS-1 and AS-3 can be frozen anytime up to their expiration date. What about AS-5? Another statement is that CP2D AS-3, AS-5 and CPD AS-3, AS-5 are not approved for rejuvination. However, non rejuvenated RBCs collected in CPD, CP2D or CPDA-1 must be frozen within 6 days of collection. Here's where I get confused...our RBC that we have, from ARC, are either AS-1 from CPD, or AS-5 from CP2D or AS-3 from CP2D. Is there a time when AS-1 and AS-3 are never collected from a whole blood from CPD or CP2D? And if not, how is it that AS-1 and AS-3 can be frozen up to their expiration dates? My understanding here is that all of our ARC units can never get frozen unless its w/in 6 days of collection. Or does that statement only refer to units with CPD, CP2D or CPDA-1 without Adsol? How can we store special antigen negative units? Thoughts or clarifications please!
I think the point of testing is is that depending on what you are using for treatment, one antigen will be destroyed and the other not destroyed. If its a known cell, K +, E - or K - E+ then yes, it would be best to run these cells non-treated at the time of reading the treated cells to prove that pos is pos and neg is neg and expected results are obtained.