aafrin

Marvy1_Actually RDPs made by PRP method may have visible red cell contamination, whereas buffy coat platelets usually have almost none or very less red cell contamination. This has been our observation since shifting the method of preparation to buffy coat.

Malcolm, thanks for the wonderful explanation. Really great.

Malcolm, Please accept my condolences,,,what a tragedy. If I may ask what happened? accident or???

I have not had any chance to meet Prof. Garratty, but have known his contributions in TM. My condolences to his family & friends. May his soul rest in eternal peace. Amen.

Here's Top Story from AABB SmartBrief dated Feb. 16. Nation's first fecal bank raises issues of research, regulation OpenBiome has been established as the first fecal bank in the U.S., providing hospitals with screened material for fecal microbiota transplant to treat patients with Clostridium difficile infections. Despite its promise, experts are reluctant to resort to FMT due to legal uncertainties. The FDA has said it regards the treatment as a biologic drug and is developing industry guidelines, but the agency is not going after doctors who perform transplants. Fecal transplant researcher Dr. Alexander Khoruts says regulatory clarity is needed to help develop more advanced treatments. The New York Times (tiered subscription model)

We are also unable to monitor low alarm for -80 C deep freezer. Would like to have feedback from anyone who is monitoring such low temp. @lph does having a policy of not monitoring low temp alarm, not cause a problem with FDA??

Yes, it is known as fecal transplantation (or bacteriotherapy)or Fecal microbiota transplantation (FMT)or stool transplant. It entails transfer of stool from a healthy donor into the gastrointestinal tract of a patient for the purpose of treating recurrent C. difficile colitis,- a complication of antibiotic therapy, that may be associated with diarrhea, abdominal cramping and sometimes fever. I believe it is quite commonly done these days.

I fully concur with you Malcolm; for I have noticed it many times that --- "Foolproof systems do not take into account the ingenuity of fools."

True SMILLER, but, if the system forces you to scan the patient's arm/ankle band, so that there is bedside printing (or whatever) it more difficult to fool the system (although, I am absolutely certain, not impossible - human beings will find ANY way of getting around things). There is a lovely saying, "This system is fool proof, but it is not idiot proof"! I fully concur Malcolm; for I have noticed it many times that --- "Foolproof systems do not take into account the ingenuity of fools."

Even docs don't understand BB. We had a pediatric registrar who was insisting on issue of O Rh negative FFP to a 3 yr old baby with B Positive blood group who had suddenly started bleeding at night and we didn't have any B group FFP in stock. He was refusing to accept AB group FFP which the tech had thawed. I had to step in & talk to consultant at 2 am & convince him for them to accept AB Group FFP.

O Rh Positive. Move to group specific as soon as blood group is done.

I seem to be having problem with reply posting, sorry. Malcolm, can you explain this further? I am lost, we have always used lower temperature for enhancing the reverse group reactions of anti-A & anti-B, but never in forward grouping.

Cliff, Thanks for the snowflakes - 3 different varieties, but I still miss last years' smashable bulbs

We do vitals pre, 15 min, 30 min and then hourly until completion of transfusion. The day care and dialysis patients who come as out-patients are requested to stay for an hour after completion of transfusion, but many do not opt to stay. The nurse takes their signature on the chart that they have declined to stay. All patients are given written instructions on what symptoms to watch for & to contact their caregiver physician immediately if they feel they might be experiencing a trxn reaction.

YES.Cliff PLEASE get the Christmas lights on to bring in the festive season....or have you planned something else this year???

Shortcuts! Don't forget the shortcuts some techs take to complete work - it's as if somebody has put a gun to their head and no one is going to come in next shift to continue further. They will shorten incubation time, not follow SOPs and give proper hand offs to incoming techs. I keep on streamlining work, make checklists and logs, but to no avail. I think with complete automation, technician errors will at least be taken care of with test procedures (hope???). I always tell everyone, at least use your basic commonsense, but then "commonsense is not very common".

We are awaiting IH1000 fully automated system from Bio-Rad - delivery expected in a couple of weeks.

Mabel, we have found that A1 lectin works better at 4 C than at RT (25 C), so keeping the tubes in refrigerator for 15-30 minutes may be helpful. Just check.

Could you please attach the level 1 & 3 competency forms here? Thanks in advance.

Congratulations on your success!

Why did you test it with A1-lectin in the first place?

We do blood group from integral segment before issue always.

Annually

Ditto here. For FFP also we use 1 mL=1.03