dawn w

IgG blocking study was performed by C.Wong at CarterBloodCare in 2001. It was published as an abstract in Transfusion. The reference is "Evaluation of a Method to Prepare IgG-Sensitized Red Blood Cells for Phenotyping" 2001- Vol.41, Supplimen:110S Outline steps are: 1. Wash red cells to be treated 3 times with isotonic saline 2. After the last wash resuspend the red cells to 3-5% 3. In a separate tube add 20 drops of the washed red cells 4. Centrifuge 1 min 5. Remove and discard supernatant, be careful not to disturb the red cells 6. Add 20 drops of IgG to the cells button 7. Incubate at room temperature for 15-30 minutes 8. Centifuge for 30 sec 9. Remove supernatant 10. Wash at least 4 times with isotonic saline 11. Perform IgG DAT on washed,treated cells. Be sure to include a 6% albumin control to verify the immunoglobulin coating has been removed. If the DAT is negative the cells are ready for typing. If the DAT is positive repeat the treatment. No more than three times. *This procedure works best for initial DATs no stronger than 2+ **This procedure can be used on EGA treated cells. ***This method peserves the KELL system antigens if used with non EGA treated cells. If you have any questions please email me at dwebber@carterbloodcare.org

I am looking for documented support to stress the fact this needs to be done daily. Any help would be greatly appreciated.

We do use PEG in place of gel crossmatching. On occasions of strong colds we do skip the IS and go straight to AHG. As to your question regarding validating AHG for detecting ABO incompatibility, we did perform mis-match ABO compatibility validation with a new vendor of PEG to demonstrated that the PEG did catch it. I admit this may not be the perfect solution to the problem, but in order to meet the standard it was the best we could come up with. Any advise would be appreciated.

Thru validation we also proved that gel picked up ABO incompatibility so in order to meet the requirement we stopped doing gel xm all together and just carried our IS thru Coombs when needed.

We have used lab assistance for the past 10 years and the help is amazing! We currently employee 10 of them and they perform inventory control, daily equipment maintenance, issue and pack blood. Pretty much all activities not related to actual testing and interpretation. I'll be happy to share a job description with you if youre interested. Drop me a line, dwebber@carterbloodcare.org

Hey Folks, For any of you looking for a reagent alternative, Quotient BioDiagnostics reagents are fabulous. We performed extensive studies and found them to perform at or above our current supplier. For information you can email me at dwebber@carterbloodcare.org

OOOUCH!!! Says my CFO :mad: Have no fear there are other options. 1. Alba Blood Group Reagents www.albabioscience.com 2. Don't for get our friends at Olympus 3. BioRad will hopefully hit our markets soon, the sooner the better

Good Morning, We are faced with the delima of meeting the AABB Standard with regards to MD participation in blood utilization for our contracted crossmatch acounts. We serve close to 100 accounts and "participation" for each will be a huge undertaking. Does anyone have any insight on how to approach this standard? We have asked for clarification from AABB, but any suggestions you can provide would be much appreciated. Thanks, Dawn