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Hello everybody, I'm new in this..uf, so Folks if you have any advice I would really appreciate it! We are establishing QC system - ALL controls concerning our reagents, ID-Cards, daily control, external control, working competence for lab Engineers. Do you have any experience in what kind of controls do you use, how often...Do you have "special testing for competence" for Engineers in lab, or it's enough if they do internal/daily/external controls? Uf, please help! Have a nice day to everybody, Sassa
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Not at all, JBP. Thanks for your remarks. Have a nice, smilling day, S.
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this is manufacturer's instructions for DCT on gel card..which we are using.. Have a nice day, Saša
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we did DAT, treating patient's cell using Liss, because it was negative we were a bit confused..
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Hello, in our lab we using Biotest Seraclone reagents and they are great. We also using Millipore reagents (monoclonal), they are also simple to use and you have results in 5 - 15 minutes. Have a beautiful day on the last day of this year and all the best to all! Sassa
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Hello Anna, Thanks for your answer. After treating red cells and also plasma, we made conclusion that it must be sth. which reacts unspecific (and not antibodies). Yes, we still do crossmatches using enzyme technic, we (not me!) didn't decided to go on "type and screen" , so I believe when you say, that we must be the last in Europe. ;-) Have any really nice day, Sasa
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Thank you Shily for your answer. We did autoadsorption, cause the patient didn't receive any transfusion, and the ICT was the same like with native plasma. We found that it might be sth. in this plasma which nespecific react with our dilution reagent (LISS) and with LISS/Coombs card (gel). When we did suspension in NaCl the autocontrol was neg. Also ICT in tube test was neg. Have a nice day, Sasa
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Hello, everybody! I have interesting specimen of pacient in our lab. Patient is A, Rh D+, we do crossmatch (gel) with two donors and we get in NaCl technic - gel (with adding bromelin) negative and in LISS/Coombs tehnic (AHG) positive. We do ICT and autocontrol and they were all positive. We do also antibody screening with test cell reagents treated with papain and antibody screening with test cell adding bromelin and there were ALL NEGATIVE!! As I mentioned before autocontrol was positive, but DAT was Negative! I don't have any idea left what to do...any suggestion? :cool: Thanks in advance! Sasa
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Hello, Paulis. I was searching something about my work on net, and I found this interesting site. Have a nice smilee day!
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Just a quick hello to everybody from me and introduction: my name is Saša and I work at Blood Transfusion Center of Slovenia (Europe), working as Lab Technition in Reference laboratory. I like reading BBT, cause I can learn new things and we can find good solution to problems together. Have a nice day! Saša
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Hello Everybody! This is my first time that I'm replaying to somebody question. First I would like to introduced my self my name is Saša and I work for Blood Transfusion Center of Slovenia (Europe), working as Lab Technition in Reference laboratory. We do elution when autocontrol and DAT is positive, especially when IgG is positive. I agree with Janet, I think if the patient hasn't got transfusion lately (or being pregnant), elution is not neccesary. S.