larevalo

Our Antibody identification in gel method(Diamed) shows an exact pattern of Anti-S.So the technician proceeds to antigen typing for S.But the phenotyping results was positive.We checked all our reagents QC,expiration and Patient's ID, all were OK.So the next step was to check for RF(rouleaux formation)There was strong RF and to asked for another sample.With the second sample the staff used tube method(Immucor) .Immediate spin shows weak reactions on some cells but negative on 4,5,7,8,15.But at 37C and AHG Phase all reactions were positive including auto control.DAT was negative, this patient received blood transfusion last 2004. Antibody screening was negative. Because there was RF we used immediate spin saline replacement technique: the results was negative. Is there any other technique to solved the problem?

In ISBT 128 labelling requirements, are blood transfusion sets included? If so how much it will cost /box?

Does anyone knows the acceptable range for critical timer? And acceptable temperature of the freezer? Thanks

To adiescast,thanks for the info. this will help me a lot in resolving the problem.

Last week I had problem with our supplier.They changed their policy not to accept our blood returned units (7 days before the expiry date)as what was agreed upon.That might increase our waste units.So the tendency is to change our policy re- returned units and decrease the standing order for each group.In applying the inventory level I follow the formula on AABB Tech Manual + the blood ordering request guidelines.The ave.no. of transfused Units/week is 8 ,so in 1 mos. is 32 units.Our blood anticoagulant are mostly Sagm(42 days exp.date)My problem is when there RTA patient comes ,most doctors here are requesting 10,10,10 for each blood components and addition of 10,10,10 for suceeding time until the patient is not stable,they will request for more...The total no.of standing blood I have is 40 units, how will I manage this if my Group O is just 14?and to request blood from our blood supplier will take max. of 2 hours to reach our facility? will you help me pls.

This doesnt concern with our facility but a research work that was assigned on me.The QC for blood components such as FPRBC :are wbc count,culture,volume,For Platelets :pH,culture, volume ..etc. I wonder if there are added QC testing for each components.Any website I can search for the up dated QC for components? Thanks

Hi ,is anyone knows the ideal speed (rcf) for eppendorf centrifuge?We are preparing for validation performance for this centrifuge .It so happened when this equipment came to our section the supplier who installed it did not explain the importance of it.From the manual it doesnt say so only the time is mention which is 10 min.Our BioEngineer says rpm.What is the difference of rpm to rcf?

Hi,Iam working in tranfusion services wherein donor Unit is not included.My director asked me to search for the updated tests done for all blood components as to AABB standards.I presented to him what I search from internet and from 15 th Ed.Technical Manual but hes still not satisfied with my research, Can anyone help me?

Our facility uses gentle heat elution in resolving problem if Rh control is positive in Rh typing.What other methods your facility used in resolving this problem?

Thank you for the information

In doing culture for platelets Is there any required volume needed to perform the test? or the same as in blood c/s?

Thank you Frank,'am going to discuss it to our medical director .

Our reference lab.does culture for all blood component as part of their QC every mos.AABB Standard says we should have a policy in detecting bacterial contamination for platelets before transfusion.In urgent cases,wherein the time is limited ,so what happen is to issue and no bacterial detection done for platelets.(we don't have automatic culture machine that will detect the presence of bacteria in platelets)Pls.teach me what to do.We are now on the process for AABB accreditation.