larevalo

To all who replied to my thread re- space requirement,thank you very much.All the knowledge,expertise you know will guide us to start the right thing in planning to have a better space.

Thanks Smiller.

Thanks HTH,you answered my question clearly.Have a nice day!

We are in a process of expansion.Is there anyone knows what is the actual space requirement the CAP requires for a hospital with 500 bed capacity? And what are the things we need to consider? Thanks in advance

I would like to know how is Turn around time applies in your transfusion services? Is it from the time we received the request along with the sample until coombs phase? or after immediate spin if the previous record shows negative antibody screening and crossmatch if any:confused:

yes ,khalidm3 i agree that we need to have negative control in reverse by using o cells.

Hi Brenda,what a big help!thats the advantage of being a member of this forum,I do appreciate to all the people who had shared and answered to my thread,thanks blood bank talk:)...P.S. anti-M is eliminated, because antibody screening is clear negative both in tube and gel method.

Hi Brenda, got it clear.Thanks. Which do you apply first in serum typing discrepancy,(under microscope read as true agglutination in combination of rouleaux) Saline replacement or anti-A1 Lectin for the presence of unexpected anti-A1 in reverse? or both?

Hi khalidm3,thanks for the info.,we do have reference lab.but before we send to them we have to find our way to rule it out .For ABO/Rh typing we are using tube method only.Both tube & gel methods are applied mainly for ABS & ABI .

hi Shily, got your response,thanks.

Thanks Donna,I think you are right .I'll let my Specialist decides which routine procedure we are going to apply.

Thanks khalidm3.

Hi shilly,thanks for your reply,only i want to clarify if you see the reaction macroscopically and the results is negative,but your microscopic reading rouleaux formation is not completely ruled-out, are you going to report this as negative?

How do you perform saline replacement when there is serum discrepancy due to rouleaux formantion?From the last step,2 drops of saline is added and resuspend it with red cells does it need to read the agglutination macroscopically?(because iso-antibodies are IgM antibodies) AABB Technical manual did not estates if microscopic or macroscopic.Any help?

QRISTOFIR,I agree with you & its in the Standard that the maximum time for blood to transfuse is within 4 hours.It didn't say if there is interruption...The discussion was about the interruption & the possiblity for bacterial contamination for the same patient since the unit is exposed to ambient temperature,for more than 30 minutes only the line has been closed during the time of transfusion and has to resume once the allergic reaction has gone.

Hi, is there anyone knows what AABB standards that specify how many hours the blood should be hanged , while waiting for allergic reaction to subside ?Because there was a discussion why for returned units of unused blood to blood bank must be < 30 minutes if kept outside the fridge,while pricked units wherein IV set is still attached to patient can still continue even more than 30 minutes?

thank you doug.

Dear Lara,It would be a big help for me if you would share your QC/QM procedures for Transfusion services because we are now on a process of complying deficiencies for AABB Standards.Thanks in advance.My email is:larevalo@hmc.org.qa

Thanks for your response,Our facility is just 150 bed capacity and most of the unit expired were mix A and O group.We keep 20 Units of O FPRBC as our standing order including those for emergency cases.We are almost 50 km away from the Donor Unit and our new director change the policy not to return the nearly expiry unit to Donor Unit,bec. he wants to monitor our new internal indicator which is the expired units.We follow the equation used in managing blood bank inventory.But to get the bench mark for expired units is still a question to me.

Thanks,the standing order of you blood components is almost the same as ours.But how you were able to got those figures for each ABO group?

Is there any method or procedures or formula in eliminating the high rate of expired units in our blood inventory.And what is the benchmark?The size of our hospital is 120 bed capacity.

thanks .

No, that's why we asked for another sample.On the first sample we were getting weak A Rh pos.(after 6x washing)The second & 3rd sample was O Rh positve .

The protocol in OBGyn Unit -Operating Theater in our hospital is to send cord blood sample after delivery for a new born screening (this include ABO/Rh and DAT).Once the baby is transferred to NICU from there, they will send again but this time the sample is from the baby and the request is the same. Is there a universal policy to send cord blood for ABO/Rh grouping?Because we encountered problems (ABO discrepancy)using cord blood sample.To confirmed the results we asked for second sample from the baby . Any comment?

Hi David,the Immediate spin technique for rouleaux formation that we do is by adding 2 drops of serum and LISS to screening cells I ,II, then centrifuge and read for hemolysis.Then we take off the serum and LISS and add 2 drops of saline.Centrifuge and record the results.