ianeke

We have the Galileo and have had so many false positives we can not use it to run panels. A smaller lab I worked at used the manual Capture-R system and had a high amount of false positives as well as false negatives when compared to Peg panels. We are considering the Provue since our techs are confident with Gel. Any feedback on Provue is greatly appreciated.

The tech manual states, "to lessen the possiblity that chance alone has caused an apparently definitive pattern, there must be a sufficient number of red cell samples that lack and a sufficient number of red cell samples that express, most of the antigens listed in table 19-1." The table refers to Rh,K,Duffy,Kidd,P,Le,MNS. How can we rely on a single example for a rule out?

I agree with this. Our facility is debating the probability issue. How many homozygous cells will confidently rule out an antibody? The 'rule of 3' would be ideal but not always practical. Only using one rule out cell would not allow for the chance that a given cell is not performing correctly and demonstrated a false negative. I have recommended using 'two or more'. Our rule out for Kell is heterozygous, as well as the C/E when anti-D is present (we suggest techs use PEG for these). I dont make the SOP and there is currently a push to reduce the rule out to one cell per antibody. What does AABB recommend? :cool:

I am a new member. I dont know how to start my first post. Thank you, Ianeke:)