Darin

Don't let a title become a trap.


"I have to be a doctor." "I need to get into that school." "I can't quit this job—it's Google."

There are many ways to live an amazing life. Stop thinking about the status of this particular title and focus on the substance of what you want. You’ll often find there are many ways to get similar amounts of joy, purpose, or freedom.

If you attach your happiness to a single path, you'll become blind to other wonderful opportunities around you. And many of them might be a better fit for you or deliver a similar result with less struggle.~ James Clear

Don't sit down and wait for the opportunities to come. Get up and make them.
~ Madam C.J. Walker

The bad news is time flies.

The good news is you're the pilot.
~ Michael Altshuler

Act as if what you do makes a difference. It does.
~ William James

The bad news is time flies.

The good news is you're the pilot.
~ Michael Altshuler

Don't sit down and wait for the opportunities to come. Get up and make them.
~ Madam C.J. Walker

It is my understanding that, while resistant, it is not totally unaffected and can even be enhanced due to cleavage.
From the internet search:
Rh Antigens and Papain: While intact Rh-positive red blood cells treated with papain may not show a change in the size of the Rh-containing polypeptides, sequential treatment with phospholipase A2 followed by papain does result in cleavage of a 35-Kd protein band to a 30-Kd band, suggesting some effect on the Rh-related polypeptides.
Increased Agglutination: Interestingly, papain treatment often leads to enhanced agglutination (clumping) of red blood cells by anti-D antibodies. This suggests that while the D antigen itself might not be entirely destroyed, the changes in the red cell membrane caused by papain somehow make the antigen more accessible to the antibody or enhance the agglutination process. One theory is that papain modifies the red cell surface, reducing its electronegativity and exposing hidden antigens. 
Here's a couple of articles discussing:
https://www.sciencedirect.com/science/article/pii/S0006497120626842
https://www.sciencedirect.com/science/article/pii/S0006497120858455#:~:text=studies%20described%20here.-,RESULTS,produce%20other%20detectable%20cleavage%20products.
Very interesting ... thank you!

It is my understanding that, while resistant, it is not totally unaffected and can even be enhanced due to cleavage.
From the internet search:
Rh Antigens and Papain: While intact Rh-positive red blood cells treated with papain may not show a change in the size of the Rh-containing polypeptides, sequential treatment with phospholipase A2 followed by papain does result in cleavage of a 35-Kd protein band to a 30-Kd band, suggesting some effect on the Rh-related polypeptides.
Increased Agglutination: Interestingly, papain treatment often leads to enhanced agglutination (clumping) of red blood cells by anti-D antibodies. This suggests that while the D antigen itself might not be entirely destroyed, the changes in the red cell membrane caused by papain somehow make the antigen more accessible to the antibody or enhance the agglutination process. One theory is that papain modifies the red cell surface, reducing its electronegativity and exposing hidden antigens. 
Here's a couple of articles discussing:
https://www.sciencedirect.com/science/article/pii/S0006497120626842
https://www.sciencedirect.com/science/article/pii/S0006497120858455#:~:text=studies%20described%20here.-,RESULTS,produce%20other%20detectable%20cleavage%20products.
Very interesting ... thank you!

The person who learns the most in any classroom is the teacher.

If you really want to learn a topic, then “teach” it. Write a book. Teach a class. Build a product. Start a company.

The act of making something will force you to learn more deeply than reading ever will.
~ James Clear

I can't recall having come across an anti-D reacting like this (as far as I can remember these days - which isn't very far at my age!!!!!), but I do remember once finding an anti-E that only reacted by IAT, and not with enzyme-treated red cells, which I sent to the International Blood Group Reference Laboratory for confirmation, and it was confirmed by no less a person than Joyce Poole. We published a poster about it at a BBTS Annual Scientific Meeting (MacRate EB, Carrington T, De Lord C, Needs M, Win N.  Anti-E Detectable by IAG and Not by Enzyme?  How Confusing!  Transfusion Medicine 2001; 11 (Suppl. 1): 30.).

What we do for ourselves dies with us - what we do for others remains and is immortal.
~ Albert Pike

If you look for evidence that people are conspiring to hold you back and the world is working against you, then it will take you no time at all to find precisely that.

If you expect to encounter helpful people and experience a world that is working with you, then you find plenty of evidence to support that view as well.

The raw material for a sweet life or a bitter life is always there. The story you emphasize is the one you notice.
~ James Clear

Thank you, you absolute legends!

Surround yourself with people who have the same goals as you.

Rise together.
~ James Clear

A real friend is one who walks in when the rest of the world walks out.
~ Walter Winchell

Fantastic song! 👍

You can choose a ready guide

In some celestial voice

If you choose not to decide

You still have made a choice

You can choose from phantom fears

And kindness that can kill

I will choose a path that’s clear

I will choose free will.
~ Rush (rock band)

It is showing pretty strongly (3-4+) on Echo.

Be happy for this moment.
This moment is your life.
~ Omar Khayyam

Thank you

Thank you❤️

I'll say! Happy B-day!

Kind words can be short and easy to speak, but their echoes are truly endless.
~ Mother Teresa

Lot's of great people born on this day 😉. Happy Birthday!🎂

Whenever you are stuck searching for the optimal plan, remember:
Getting started changes everything.
~ James Clear