mondayw

Hi Exlimey, this makes sense. Thank you for taking the time to explain it thoroughly. I appreciate it!

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Hi Bet’naSBB, That’s the thing I’m confused though, how could we ID it as a warmauto if AC was negative? Also, was the elution necessary to perform since patient’s tube and DAT were negative since it was first identified? Is the elution result valid even if my DAT was negative? Patient was transfused years ago as well. Thank you!

Hi Yanxia, thank you for your recommendation. I did not know that you can actually do a solid phase crossmatch until now. However, it is not a part of our SOP in our lab. Might just try it next time and see how it works.

We use Lumena (solid phase) for the ID. Crossmatch is tube method IS, LISS 37C, IgG

At first I thought it might have been a high frequency antigen but it didn’t make sense as my crossmatches were compatible. I also thought it might be a cold antibody that reacts on IgG? I’m not very knowledgable on how cold antibody works and can’t back up how I came up with that 😅

Patient is identified as having “warmautoantibodies” in our lab with transfusion instruction to give C, E, K neg units. Patient was phentotyped for RH and Kell on the first work-up. When patient came back for a type and screen, 2 cell screen were 2+, ABID were all 1+ except for one cell being 3+. DAT was negative and when I performed tube screen and autocontrol, they’re both negative. When I looked at the previous work-up, no transfusion has been done since 2018, screen and ABID also have panagglutination reactions. DAT and Tube screen were also negative. My supervisor asked me to do an elution and it came out with panagglutination reaction (3+ and 4+). Since patient also needed blood, I IgG crossmatched 4 units of C, E, K neg and they were all compatible. Our facility called it as warm autoantibody but it just didn’t sit well with me. Should I have done further testing to rule out if that one 3+ meant something else on the ID? Thank you!

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Thank you for the warm welcome everyone! I’m a silent reader of this forum since this year and just decided to create account. I’m a generalist tech for over 6 years now but have started Blood Bank in 2022. I hope to continue learning from everyone’s experiences and thanks for making such an awesome forum where people working in BB could get insight and help each other out.