Lucky Jack

I'm pretty sure Quotient introduced their immediate spin monoclonal Anti-Fyb about a year ago. Grifols' Anti-Fyb is also immediate spin and was introduced when they rolled out their conventional immunohematology reagents a little less than a year ago. I haven't any of these reagents at this point but was considering it.

For those using monoclonal Duffy antisera reagents, what has your experience been? I know in the US Quotient then Grifols and now Siwa Biotech have introduced monoclonal immediate spin Anti-Fyb reagents and Siwa Biotech also offers a monoclonal immediate spin Anti-Fya reagent.

We use the following reagents from Quotient: complement control cells; A1 ,A2, and B cells; Antisera for Fya, M, N, Lea, and Leb; and their 16 cell panel The only issue I've had with them is a recall of Anti-N last year that left it backordered for ~4 months. Their complement control cells seem to produce stronger reactions than Immucor's in my experience.

In this process how is the saline physically added (sterile docked, spiked, syringe)? I've pooled cryo but never with saline. When I was on the hospital side we did get pre-pooled cryo from our blood supplier but still occasionally pooled when a provider insisted on a number of units that wasn't a multiple of 5.

Anyone using the Hettich EBA 280?

I just answered this question. My Score FAIL

I have a question regarding DTT treatment of plasma - I understand that if there is reactivity persistent in the saline control and absent in the DTT treated plasma, it indicates that reactivity is caused by an IgM antibody. What steps, if any, can be taken to show there wasn't also an IgG antibody that was diluted to the point of undetectability in both the DTT treated plasma and the saline control plasma dilution? Or am I just thinking about this wrong?

Just curious, do you (or do you plan to) do calibration of this thermometer in house, send it out to be performed, or replace the equipment when the cal expires?

In what situations would you use a saline replacement technique if the apparent reactivity is suspected to be only rouleaux?

The Last Chance Review is now called PASS. It looks like handouts for the 2018 session are available for purchase at http://www.passbbexamreview.org/

For those of you who dilute 3-5% reagent RBC solution to a 0.8% solution what dilution process do you follow? what volumes of 3-5% suspension or packed RBCs and diluent do you use? The Ortho/MTS instructions for use gives the example of 10µL of packed RBCs in 1.0mL of diluent but leaves it open to other processes and preparing 1.0mL of cell suspension per reagent RBC tested seems excessive.

None of the institutions I have have worked for have added check cells to the DAT control - the check cells should be QC'd as part of daily reagent QC. From my point of view, since no AHG is added to the DAT control, the check cells serve no purpose. For negative Poly, Anti-IgG, and Anti-C3 DATs we did add check cells to verify that those reagents were not neutralized by free IgG or complement.

Thank you!

Hello, Does anyone happen to have a digital copy of the user/operator's manual for the old Ortho MTS gel incubator (Model# DG-225) that they would be willing to share? It's the tan colored one that sits on top of the centrifuge. Many thanks,