
Everything posted by Arno
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Positive Antibody screen but negative antibody ID panel
Yes indeed different pH, different suppliers may explain such a behavior (some anti-M are enhanced with acidification of plasma). In addition, Anti-M often shows dosage effect but I believe you have antigen M double dose cells on your panel too. What are the phenotype of the 2 cells reacting in screening and the one not reacting? Is your patient antigen M negative? It also exists the anti-M1 (the M1 antigen belongs to the MN CHO collection) that reacts with some M positive cells and stronger with M/N positive cells (M1 is expressed on M positive cells) and it can be, though rarely, produced by antigen M positive patients.
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Unidentified Antibody, Little Help Please
First of all, if the cassette Ctl is positive, the blood type result is invalid (esp. the D antigen typing) . Looks like a (warm) AIHA and several rounds of adsorption (allo with enzyme treated cells or auto, depends on date of previous transfusion, how much RBCs are available and possibility to "clean them up" using ZZAP for instance) may bring some clarity here to check if there is an underlying antibody.
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Positive Antibody screen but negative antibody ID panel
In addition to what has been nicely explained by Malcolm, it could be as well an example of Sd(a++) cell (commonly named "super Sid") reacting with a weak anti-Sda. The Sda antigen is not a LFA (expressed on more than 90% of cells) though some cells "overexpresse" it. Anti-Sda usually gives weak/DP reactions and can be neutralized using urine (contains soluble Sda substances). Other weak antibodies may behave the same way, e.g. anti-P1 reacting against "strong P1" cells only. However, that does not change at all what Malcolm said "I wouldn't expend too much time or energy trying to sort out the exact specificity. In all cases of such an antibody, as long as you cross-match by the same method as you used in detecting the presence of the antibody in the first place, it would be quite safe to give cross-match compatible blood."
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BloodBankTalk: Revised Recommendations for HIV Transmission through blood transfusion
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Anti-Viral interference
Hi, Not aware of such kind of interference with nucleotide analog. It would be more likely if it was with convalescent plasma.
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Daratumumab patients positive with DTT treated cells
In which buffer do you resuspend your DTT treated cells? May be these patients do have antibodies against one or several components of this buffer (antibodies against preservatives used in RBC buffer are not so uncommon).
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Antibody stimulation by antigen negative blood?
Hi Rich, I am not a clinician but as far as I know IVIG can be given to obstetrical patient in diff. conditions (autoimmune disorders, recurrent pregnancy loss, ...). I thought about IVIG when I saw the DAT becoming positive plus additional reactions coming up over the time. Anti-A and Anti-B are indeed the most prevalent antibodies in plasma derived products but other specificities of low titre can be present sometimes such as anti-D, anti-K and a bunch of antibodies of undetermined specificity reacting with several to not say all RBCs. Just a thought that can be doublechecked with the clinician..? Hereunder is a very great (not recent though) paper to be read and re-read again: Problems Associated With Passively Transfused Blood Group Alloantibodies George Garratty, PhD, FRCPath American Journal of Clinical Pathology, Volume 109, Issue 6, 1 June 1998, Pages 769–777, https://doi.org/10.1093/ajcp/109.6.769
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Antibody stimulation by antigen negative blood?
Is she given plasma derived product (not talking here about anti-D prophylaxis)? Thinking more here about IVIG?
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BloodBankTalk: Antibody/Antigen Reaction
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BloodBankTalk: Allergic Reaction
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Blood Group Discrepancy
The reaction with the A2 cell would show there is an anti-A (+an anti-A1) so I presume it would rather speak for a B(A). I would expect for a AweakB having an anti-A1 only (?).
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Blood Group Discrepancy
Could it be a B(A) phenomenon with elevated level of B transferase and decreased activity of the anti-A (and anti-A1)?
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cord blood
I believe newborn and maternal red blood cells do not have exactly the same density. So, on 2 different sampling even from the same tube of packed cells, you may have diff. proportions of maternal vs newborn red blood cells. It is the same in case of transfusion, as transfused cells are heavier, depending on the way RBCs are sampled (bottom/middle/top of packed cells) you may have diff. results/pictures (DP, no DP...).
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BloodBankTalk: Antibody/Antigen Reaction
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ABO TYPING DISCREPANY WITH MTS GEL
If the reverse cells are ficin treated, it might be the anti-c giving this mf result.
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DARALEX/DARATUMUMAB PATIENTS
https://www.ncbi.nlm.nih.gov/pubmed/30779172 Interesting method developed in Brazil if you get access to the drug.
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BloodBankTalk: Antibody/Antigen Reaction
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BloodBankTalk: Correct Blood Bank Nomenclature
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BloodBankTalk: Clinical Aspects of Transfusion Reactions
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ABO diacrepancy
To my knowledge no (?). I believe it is more likely due to his disease leading to this hematopoiesis stress and may have evolved over the time (?).
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ABO diacrepancy
Diseases leading to hematopoiesis stress may influence (i.e. weakening) the A, B, H and I antigens expression (less binding of carbohydrate chains). How is his reverse group?
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Anti-G
Some good materials here as well => https://www.bbguy.org/2016/06/17/want-g-wiz/
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BloodBankTalk: Antibody/Antigen Reaction
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BloodBankTalk: Clinical Aspects of Transfusion Reactions
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BloodBankTalk: Allergic Reaction
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