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🌟 Join Our Immunohematology Community! 🌟 Peace and blessings be upon you all. I’m excited to invite you to join my Telegram group Immunohematology Made Easy – a space dedicated to learning and sharing knowledge about blood transfusion medicine. Whether you're a beginner, an experienced professional, or an esteemed expert, there's something for everyone in our group! In this community, we focus on: Learning Together: From the basics to advanced concepts in blood transfusion, we’re here to learn and share. Open Discussions: Ask questions, share insights, and help simplify complex topics. Supportive Environment: Everyone’s contribution matters, regardless of your level of experience. This group is a platform where: Newcomers can learn at their own pace. Experienced professionals can share their expertise. Everyone can join in discussions and grow their understanding of this critical field. Let’s connect, grow, and contribute to advancing transfusion science together! If you're passionate about this field, feel free to join and invite others who might benefit. 📲 Join us: Immunohematology Made Easy group

Of course, my facility is blocking the link..... Is this a web page? Could you private message me the link please? TIA

For decades we hired only MTs, but have had to hire a few MLTs over the last couple of years due to MT shortages. I find the quality of work varies not from the number of college courses they took but the innate ability, initiative and interest of the worker. We train equally and job responsibilities are equal (although the MTLs cannot do some things, like review results etc). I had a MLT generalist on last weekend. He had a patient who got 4 units of blood the week before who now presented with anti-c and a positive DAT, weak mixed field. Pretty classic delayed reaction, except that the eluate reacted with all the panel cells. DAT was negative the week before. He dug into it and tested some more c-negative cells and found that there was also anti-Fya and -Jkb in the eluate (but not in the plasma yet). En route he tested the eluate with ficin-treated cells and PeG and ficin-treated some additional ones himself to help untangle the specificities. I did give him some phone coaching along the way, but it was an excellent job of blood banking. "Just" a MLT, but he really digs BB.

I use the recipes below for students/new techs. We use mostly gel for testing, and these work pretty well most of the time. The Rh, K, and M are very consistent and we get 2+ reactions or better. I don't know what kind of reactions you would get with tube testing with the Rh and K. I've had problems with the Fy, so I only use it in combination with other antibodies. Sometimes I mix it up based on what our expired panels look like. If I'm feeling particularly evil (or want to stump a student who thinks they can solve anything), I will choose several antibodies that are very difficult to identify with a particular panel. We use mostly monoclonal antisera, and I try to use expired, but since I'm only using a drop or two, I don't feel bad about using in-date reagents. I either match the antibodies to an expired panel cell to use as "patient" cells, or use some segments from a phenotyped unit if one is available. I've tried making positive DATs with reagents other than D, but haven't had much success. Elution: Anti- D, mixed field DAT 2 O Pos Segments and 2 O Neg segments Put O pos segments in 12x 75 tube. Add 1 drop of Anti-D, 2-3 drops of albumin and a little bit of saline Incubate at 37 for 30 minutes Add O neg segments [*]Gel ab screen- Anti- C Fill 12x75 tube 1/3 full of saline Add 1 drop Anti-C, 2 drops albumin [*]Gel ab screen- anti-Jka Fill 12x75 tube 1/3 full of saline Add 1 drop Anti-Jka, 2 drops albumin [*]Gel ab screen- anti-K and Anti- c Fill 12x75 tube 1/3 full of saline Add 1 drop Anti-K, 1 drop Anti-c, 2 drops albumin [*]Tube Ab screen – Anti-M Fill 12x75 tube 2/3 full of saline Add 2 drops Anti-M, 2 drops albumin [*]Gel Ab screen - Anti-Fya, Anti-E, Anti-K Fill 12x75 tube 1/3 full of saline Add 2 drops Anti-Fya, 1 drop Anti-E, 1 drop Anti-K, 3 drops albumin