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Due to the recurrence of requests for Direct Antiglobulin Test (DAT) and, if positive, to perform the Eluate for patients with suspected Autoimmune Hemolytic Anemia (AIHA), I come here in the hope of obtaining answers or ideas from the specialists in this group. I understand the purpose of performing DAT (IgG/IgA/IgM and C3) to confirm cases of clinical suspicion of AIHA and/or to monitor an ongoing hemolytic process. But I wonder: What additional information can be obtained from the elution of red blood cells where IgG has already been detected? I understand the importance of the Eluate in the transfusion context, but knowing the methodological limitations and risks of false negatives, I cannot understand how doctors are interpreting and defining treatment based on this test. Is it possible to confirm a clinical suspicion of AIHA using immunohematological tests without performing the eluate? My opinion is that yes, based on some references. My interpretation is that the eluate would be important in the laboratory diagnosis of AIHA only if DAT is negative. I appreciate contributions.