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Way back in 2003, the AABB published "Guidelines for Implementing an Electronic Crossmatch". In the section REQUIREMENTS on page 4 "Additional criteria suggested, but not universally accepted, include: ... The antibody screen should include a three- or four-cell sample." No further explanation was given but presumably it was for the reasons stated above that you are likely to have more cells with a homozygous antigen expression in a 3-cell panel than you are in a 2-cell panel. Most manufacturers of 3-cell panels make sure to include cells that are homozygous for Duffy and Kidd to avoid missing those. So long story short....that's why we use a 3-cell panel as part of our due-diligence of providing blood by computer assisted crossmatch.

I performed this study of long term stability (14 days) of panel cells treated in bulk as my SBB project. I also used regular blood bank saline vs the 8.0 from the technical manual. I was able to publish my study and findings in the Immunohematology journal. I would be happy to share if you leave your email address. Happy to help those interested.

We added a note in our labeling policy what the truncated name would look like, ie a name more than 35 characters would be truncated at 35 characters and have a plus sign. We compare as much of the truncated name as possible, the unique BB identifier number, and another identifier like mrn or DOB.