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On p. 563 of AABB Tech Manual 18th edition, it only mentions that titer methods other than "saline AHG 60 minute incubation" in tube may result in higher titers and "should be validated with clinical findings" (see Malcolm's post, above). So it does not seem to say one cannot use gel or other methods, just that you need to document validation. I have always been a bit uncomfortable with identifying an antibody with gel (for a prenatal), then doing the titers in tube. But then again, I guess it is the comparison of the series of tube titers that they are looking at. Scott

Nice work, congrats! When I was doing my SBB training our reference lab manager/educator had an running bet, she'd buy dinner for any student that took an SBB exam and didn't get a question about anti-D,C and G. Don't think she has bought anyone dinner yet