ESIZENSKY Posted December 18, 2018 Share Posted December 18, 2018 I am validating DAT's in the gel method (Biorads). I have several DAT's (Cord) positive in tube, confirmed LUI freeze ABO antibodies) negative in gel. Has anyone had this problem when validating in gel. Link to comment Share on other sites More sharing options...
noelrbrown Posted December 20, 2018 Share Posted December 20, 2018 Which gel are you using and what is the source difference for the anti IgG i.e. monoclonal versus polyclonal or is it anti complement issue you are experiencing? Link to comment Share on other sites More sharing options...
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