ifedajay Posted July 31, 2018 Share Posted July 31, 2018 Hi guys, I really need some help with setting up the DTT for DARA patients protocol at my hospital. 1. Which reagent do you use? We prefer the prepared 0.2 M DTT. Which manufacturer do you buy from? 2. Could I get a peek at your SOP? 3. Do you use single-use DTT preparations to repeat the antibody screen? Do you instead prepare a bunch each month and preserve in Alsever's solution? 4. Is it ok, to just repeat only the antibody screen and not do the screen and panel? 5. Any other tips/advice are MUCH appreciated! :) Sincerely, Gloria N. Link to comment Share on other sites More sharing options...
David Saikin Posted August 2, 2018 Share Posted August 2, 2018 On 07/31/2018 at 9:55 AM, ifedajay said: Hi guys, I really need some help with setting up the DTT for DARA patients protocol at my hospital. 1. Which reagent do you use? We prefer the prepared 0.2 M DTT. Which manufacturer do you buy from? 0.2M DTT purchased from Hemobioscience. 2. Could I get a peek at your SOP? I only have a rudimentary one. I was setting this up when I took a LOA and now my BB does no advanced studies at all. 3. Do you use single-use DTT preparations to repeat the antibody screen? Do you instead prepare a bunch each month and preserve in Alsever's solution? These patients are very rare. We do one case at a time. The reagent DTT may be thawed up to 5x. 4. Is it ok, to just repeat only the antibody screen and not do the screen and panel? that is up to you and your medical director. 5. Any other tips/advice are MUCH appreciated! Follow the protocol as provided by Hemobioscience. My experience is that if you do not, you procedure will fail. Sincerely, Gloria N. See my answers in between your questions. Link to comment Share on other sites More sharing options...
David Saikin Posted August 2, 2018 Share Posted August 2, 2018 I tried to send you a message with my procedure. Send me one and I'll forward it to you. Link to comment Share on other sites More sharing options...
carolyn swickard Posted August 3, 2018 Share Posted August 3, 2018 (edited) DTT SOP.pdf This procedure is based on the HemoBioscience SOP and the AABB SOP. Works for us. Prior threads on this topic have indicated that the DTT treated cells will not last long, so we do this only at need. Edited August 3, 2018 by carolyn swickard message AMcCord, David Saikin and gagpinks 3 Link to comment Share on other sites More sharing options...
ifedajay Posted August 3, 2018 Author Share Posted August 3, 2018 thank you!! Link to comment Share on other sites More sharing options...
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