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PEG vs LISS


saralm88

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Hi friends!

I just wanted to get a feel for what different labs are using.  We have the Echo and Neo (new) and have been using LISS for screens and xm.  I am switching to using PEG because Immucor says it is the best to use when having the machines.  What does everyone else use out there? 

I hope everyone is having a great summer!  I look forwards to hearing from you - hopefully I can get on here more and give more input of my own! :)

Thanks!

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21 hours ago, saralm88 said:

Hi friends!

I just wanted to get a feel for what different labs are using.  We have the Echo and Neo (new) and have been using LISS for screens and xm.  I am switching to using PEG because Immucor says it is the best to use when having the machines.  What does everyone else use out there? 

I hope everyone is having a great summer!  I look forwards to hearing from you - hopefully I can get on here more and give more input of my own! :)

Thanks!

Hello. We also use the Echo (Liss) as primary method, and PEG tube testing as secondary method. For crossmatch through Coombs, we use PEG as potentiator.

Forgive me, for I have been in the business 3 decades next month.  The use of current terminology, I am still working on.

cottonball

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22 hours ago, saralm88 said:

Hi friends!

I just wanted to get a feel for what different labs are using.  We have the Echo and Neo (new) and have been using LISS for screens and xm.  I am switching to using PEG because Immucor says it is the best to use when having the machines.  What does everyone else use out there? 

I hope everyone is having a great summer!  I look forwards to hearing from you - hopefully I can get on here more and give more input of my own! :)

Thanks!

PEG-IAT is arguably the most sensitive tube test currently in widespread use. For this reason you're more likely to see concurrence with your Echo/Neo results if you use PEG for supplementary testing, i.e., the sensitivity of the two assays are perhaps the closest (LISS being less-sensitive). However, there's still a chance that the Echo/Neo will detect something that is not detected in PEG (or LISS).

I wonder what Immucor would say if you decided to use another manufacturer's PEG reagent ?;)

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We have had the Echo since 2009 and have found that PEG is our go to tube method. It has worked well for the questionable nonspecific iffy capture results. PEG is positive when we repeat the screen and it truly is a clinically significant antibody but negative most times confirming our thoughts that there was really nothing there in the first place.

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14 hours ago, AMcCord said:

Our primary method is the Echo. I use PeG for tube testing, with LISS available for working with warm autoantibodies that love PeG and solid phase too much.

Isn't it fascinating that we're "allowed" to deliberately use a less-sensitive assay when "we" feel it appropriate?:) Offhand, I can't think of anything similar in other path disciplines. Anyone ? Anyone ?

And..... go........

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You use a less sensitive assay method to reduce interference with cold and warm auto antibodies that you have shown or proven are not clinically significant, with the idea that a clinically significant allo antibody like Kell, if present, will shine through.  It's a balancing act.  

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58 minutes ago, Johnv said:

You use a less sensitive assay method to reduce interference with cold and warm auto antibodies that you have shown or proven are not clinically significant, with the idea that a clinically significant allo antibody like Kell, if present, will shine through.  It's a balancing act.  

Thank you, Johnv. I know the science.

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6 hours ago, exlimey said:

Isn't it fascinating that we're "allowed" to deliberately use a less-sensitive assay when "we" feel it appropriate?:) Offhand, I can't think of anything similar in other path disciplines. Anyone ? Anyone ?

And..... go........

That's why Chemists won't come near Blood Bank -scares the pants off of them.

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We use Ortho gel for screens both manually and automated(Vision). If we need to use tube method we go to PEG or 30 minute saline.

> PEG: if the gel screen is weak, doesn't make any sense, shows mix field (often seen with the burn patients)

>Saline: warm autos ( a gel screen is performed too, just to keep an eye  on reaction strength)

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