Sherif Abd El Monem Posted July 14, 2018 Share Posted July 14, 2018 my question about using saline instead of diluent ( in cell suspension preparation )in blood grouping and cross match for Diamed gel cards can this affect test results or not ? Link to comment Share on other sites More sharing options...
Yanxia Posted July 15, 2018 Share Posted July 15, 2018 yes, it can. Because the PH of the salin is in larger span than the diluent which maybe PBS .And the reaction's best PH is in the diluent. Link to comment Share on other sites More sharing options...
galvania Posted July 15, 2018 Share Posted July 15, 2018 It's not so much the pH as the osmolarity. With saline cells can 'hang' in the gel. Link to comment Share on other sites More sharing options...
Sherif Abd El Monem Posted July 15, 2018 Author Share Posted July 15, 2018 5 hours ago, galvania said: It's not so much the pH as the osmolarity. With saline cells can 'hang' in the gel can it give false +ve ? Link to comment Share on other sites More sharing options...
SMILLER Posted July 15, 2018 Share Posted July 15, 2018 ...or false negatives, which would be even worse. You would need to have something from Diamed to allow the use of non-standard reagents, diluents, etc. Scott Link to comment Share on other sites More sharing options...
tcoyle Posted July 16, 2018 Share Posted July 16, 2018 On 7/15/2018 at 6:33 AM, SMILLER said: ...or false negatives, which would be even worse. You would need to have something from Diamed to allow the use of non-standard reagents, diluents, etc. Scott And then a comprehensive validation to show that it would work in all situations. Best to stick with what the manufacturer requires in their directions. SMILLER, Baby Banker and David Saikin 3 Link to comment Share on other sites More sharing options...
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