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bacterial contamination of frozen component

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If we prepare FFP or cryo in an open system then freeze this storage temperature (frozen ) can eliminate any bacterial effect may be occurred  during processing ?

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For most species,  freezing (even in liquid phase liquid nitrogen (-196C) will not kill the bacteria.  There is a lot of literature concerning this subject for cell processing for BMT and I have personal experience with Staph species and Strep species being alive and perfectly happy in a thawed stem cell product.  Even after lengthy storage at <-150C.

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On 7/12/2018 at 4:35 PM, applejw said:

For most species,  freezing (even in liquid phase liquid nitrogen (-196C) will not kill the bacteria.  There is a lot of literature concerning this subject for cell processing for BMT and I have personal experience with Staph species and Strep species being alive and perfectly happy in a thawed stem cell product.  Even after lengthy storage at <-150C.

Thanks

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On 7/12/2018 at 5:15 PM, David Saikin said:

Your product would have a life of 4 hrs upon thawing.  The extreme cold is not bacteriocidal.

Thanks

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