richj Posted June 22, 2018 Share Posted June 22, 2018 Hello What are the pros and cons of using IgG vs AHG when performing a tube crossmatch or tube screen assuming that the tube method is a back up method to MTS gel. Is it possible to miss a complement binding IgM antibody early on by using IgG only. The standards seem to indicate that IgG only is required. Thanks. Richard Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted June 22, 2018 Share Posted June 22, 2018 It is possible, but highly improbable. Link to comment Share on other sites More sharing options...
exlimey Posted June 25, 2018 Share Posted June 25, 2018 On 6/22/2018 at 3:22 PM, richj said: Hello What are the pros and cons of using IgG vs AHG when performing a tube crossmatch or tube screen assuming that the tube method is a back up method to MTS gel. Is it possible to miss a complement binding IgM antibody early on by using IgG only. The standards seem to indicate that IgG only is required. Thanks. Richard Does the MTS gel card you typically use contain polyspecific antiglobulin reagent (anti-IgG + anti-complement) or does it just contain anti-IgG ? I think most users are using anti-IgG cards, and if that is the case, they're already dealing with the "Is it possible to miss a complement binding IgM antibody early on by using IgG only." issue. SMILLER and AuntiS 2 Link to comment Share on other sites More sharing options...
NicolePCanada Posted June 26, 2018 Share Posted June 26, 2018 Poly Specific AHG is less expensive. AuntiS 1 Link to comment Share on other sites More sharing options...
AMcCord Posted June 29, 2018 Share Posted June 29, 2018 You can avoid some annoying clinically insignificant cold reactive antibodies by using anti-IgG. Malcolm Needs 1 Link to comment Share on other sites More sharing options...
Dansket Posted June 29, 2018 Share Posted June 29, 2018 Stopped using Polyspecific AHG in lieu of Monospecific Anti-IgG for antibody screen/crossmatch tube testing in 1980 and of course Anti-IgG Gel testing since 1996. No occurrence since then that would cause me to reverse those decisions. AMcCord, exlimey and Malcolm Needs 3 Link to comment Share on other sites More sharing options...
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