Jump to content

Recommended Posts

Hello.  Does anyone ever use a 4:1 (serum to cells) ratio when investigating antibody IDs?  If so, do you use enhancement and/or have an SOP? Just looking for documentation to confirm it as a valid procedure.  Thank you.

Share this post


Link to post
Share on other sites


Only when using standard laboratory saline to suspend the red cells, and yes, we have an SOP for this (as for ALL techniques we use).  If we are using LISS, however, it is vital that a 1 to 1 ratio of serum/plasma to red cells is used, to prevent either false positive or false negative reactions, as anything else would change the overall ionic strength of the reactants in the test.

Share this post


Link to post
Share on other sites

The package inserts for your enhancement medium will tell you if it is acceptable to use more plasma/serum. As Malcolm says, no for LISS.

Share this post


Link to post
Share on other sites

One of the classic ways of increasing sensitivity is to increase the serum:cell ratio.  Used to do that a long time ago.  I've never done that with enhancement media.  I don't know if they still do but I believe Canada used to use 10 drops of serum/drop of cell suspension.

Share this post


Link to post
Share on other sites
21 hours ago, Okie said:

Hello.  Does anyone ever use a 4:1 (serum to cells) ratio when investigating antibody IDs?  If so, do you use enhancement and/or have an SOP? Just looking for documentation to confirm it as a valid procedure.  Thank you.

Not for atypical allo-antibody IDs.

Scott

Share this post


Link to post
Share on other sites

Create an account or sign in to comment

You need to be a member in order to leave a comment

Create an account

Sign up for a new account in our community. It's easy!

Register a new account

Sign in

Already have an account? Sign in here.

Sign In Now

  • Advertisement

×

Important Information

We have placed cookies on your device to help make this website better. You can adjust your cookie settings, otherwise we'll assume you're okay to continue.