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Okie


Okie

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Hello.  Does anyone ever use a 4:1 (serum to cells) ratio when investigating antibody IDs?  If so, do you use enhancement and/or have an SOP? Just looking for documentation to confirm it as a valid procedure.  Thank you.

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Only when using standard laboratory saline to suspend the red cells, and yes, we have an SOP for this (as for ALL techniques we use).  If we are using LISS, however, it is vital that a 1 to 1 ratio of serum/plasma to red cells is used, to prevent either false positive or false negative reactions, as anything else would change the overall ionic strength of the reactants in the test.

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21 hours ago, Okie said:

Hello.  Does anyone ever use a 4:1 (serum to cells) ratio when investigating antibody IDs?  If so, do you use enhancement and/or have an SOP? Just looking for documentation to confirm it as a valid procedure.  Thank you.

Not for atypical allo-antibody IDs.

Scott

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