Okie Posted February 7, 2018 Share Posted February 7, 2018 Hello. Does anyone ever use a 4:1 (serum to cells) ratio when investigating antibody IDs? If so, do you use enhancement and/or have an SOP? Just looking for documentation to confirm it as a valid procedure. Thank you. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted February 8, 2018 Share Posted February 8, 2018 Only when using standard laboratory saline to suspend the red cells, and yes, we have an SOP for this (as for ALL techniques we use). If we are using LISS, however, it is vital that a 1 to 1 ratio of serum/plasma to red cells is used, to prevent either false positive or false negative reactions, as anything else would change the overall ionic strength of the reactants in the test. Link to comment Share on other sites More sharing options...
AMcCord Posted February 8, 2018 Share Posted February 8, 2018 The package inserts for your enhancement medium will tell you if it is acceptable to use more plasma/serum. As Malcolm says, no for LISS. Link to comment Share on other sites More sharing options...
Okie Posted February 8, 2018 Author Share Posted February 8, 2018 Do you have references for your SOP? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted February 8, 2018 Share Posted February 8, 2018 Unfortunately, I no longer work for NHSBT and so I no longer have access to the SOP. Link to comment Share on other sites More sharing options...
David Saikin Posted February 8, 2018 Share Posted February 8, 2018 One of the classic ways of increasing sensitivity is to increase the serum:cell ratio. Used to do that a long time ago. I've never done that with enhancement media. I don't know if they still do but I believe Canada used to use 10 drops of serum/drop of cell suspension. Link to comment Share on other sites More sharing options...
SMILLER Posted February 8, 2018 Share Posted February 8, 2018 21 hours ago, Okie said: Hello. Does anyone ever use a 4:1 (serum to cells) ratio when investigating antibody IDs? If so, do you use enhancement and/or have an SOP? Just looking for documentation to confirm it as a valid procedure. Thank you. Not for atypical allo-antibody IDs. Scott Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted February 8, 2018 Share Posted February 8, 2018 Why not Scott? It was standard practice back in the day, and we didn't kill too many people, even then! John C. Staley 1 Link to comment Share on other sites More sharing options...
David Saikin Posted February 8, 2018 Share Posted February 8, 2018 20 minutes ago, Malcolm Needs said: Why not Scott? It was standard practice back in the day, and we didn't kill too many people, even then! That's the truth. Link to comment Share on other sites More sharing options...
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