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How do you perform quality control for new screen cells in antibody detection?


mrkeramati

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In the US, at a minimum, you must follow the manufacturer's instructions regarding QC and any other required validation.  We run QC material on all reagents once a day whether there is a lot change or not.  When a new lot number is put into use, it is QC'd first before using for patients. 

We do NOT do any sort of lot-to-lot correlation.

Scott

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We run QC once per day with known positive reagent antisera. If you’re referring to lot to lot comparison, we do not currently do that, but you could use a patient sample with a known positive screen or a neg sample spiked with a couple drops of anti D, as suggested above. 

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We dilute up some anti-D and use an aliquot of that to crosscheck the newly received antibody screening cells with the screening cells currently in use.  When the cells are put into use, we check all three cells using anti-D or anti-c diluted up so that we are testing all cells for something positive.

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  • 1 year later...

We use a commercial QC kit. The positive control is a blend of Rh antibodies that will give a positive reaction with all three screen cells. You could make something like that by spiking a negative plasma sample with anti-D and anti-c. For a negative control, we use a patient sample that was testing in the previous 2-3 days and was found to be non-reactive (negative antibody screen).

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