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dothandar

Polyagglutination

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20 months old patient with respiratory problem. Patient is O+ and antibody screen is negative by gel method at the hospital. Lectin panel was ordered at our lab. Here is our results.

Arachis Hypogea - Patient's cells 4+, Neuraminidase treated cells 4+, Pooled O cells negative

Glycine Soja - Patient's cells neg, Ficin treated cells 4+, Pooled O cells negative

Dolichos biflorus- Patient's cells neg, A1 cells 4+, pooled O cells negative

Salvia Sclarea- Patients cells neg, ficin treated A cells 2+, pooled O cells negative

Pooled adult AB plasma- Patient's cells neg, Neuraminidase treated cells 4+, Pooled O cells negative: test with 6 more sources of AB pooled plasma, all negative with patient's cells and positive with neuraminidase treated cells. 

Pooled cord AB plasma- Patient's cells neg, pooled O cells negative.

Based on above reaction, I can assume this patient has weak form of T polyagglutination, Tk, Th or Tx but I do not know why patient's cells are not reactive with adult plasma. Any thought? Should I incubate at room temp longer ? 

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Weird.....Why did they order the lectin work up anyway?  doesn't really look like a polyagglutinable sample from those results - but i don't have very much experience with lectins

I think I would be inclined to do an antibody screen in the cold (thinking auto-anti-I or -i)

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