Ficin Posted April 10, 2017 Share Posted April 10, 2017 Good Morning!! Looking for some insight from blood bankers........ I have noticed that procedures vary by institutions for the prewarm technique. Some facilities use potentiators (PEG, LISS) with the prewarm method, some do not use any potentiators for the prewarm method. The Technical Manual does not say either way. Any thoughts?? Thanks, Ficin Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted April 10, 2017 Share Posted April 10, 2017 NHSBT Reference Laboratories in the UK use LISS with the pre-warming technique, but, just occasionally, if this does not "get rid" of the "cold" antibody, we will use NISS. exlimey 1 Link to comment Share on other sites More sharing options...
exlimey Posted April 10, 2017 Share Posted April 10, 2017 (edited) One can use any of the manual tube techniques, as long as one is comparing apples to apples - if one's basic (normal) test uses a potentiator, one must use it in the pre-warmed verison. As Malcolm suggests, sometimes a plain vanilla saline antiglobulin test is the way to go, provided the "cold" reacts in that phase. Edited April 10, 2017 by exlimey Typo/clarification Malcolm Needs and Ensis01 2 Link to comment Share on other sites More sharing options...
AMcCord Posted April 10, 2017 Share Posted April 10, 2017 I try to avoid using it if at all possible. catchmenow51 and John C. Staley 2 Link to comment Share on other sites More sharing options...
David Saikin Posted April 10, 2017 Share Posted April 10, 2017 I routinely used PeG in tube testing so I used when using prewarmed techniques. Link to comment Share on other sites More sharing options...
SMILLER Posted April 10, 2017 Share Posted April 10, 2017 In general, we do not use potentiators if we go to a prewarm. Scott MaryPDX 1 Link to comment Share on other sites More sharing options...
Sherie Bruns Posted April 14, 2017 Share Posted April 14, 2017 We do not use potentiators during a prewarmm. Link to comment Share on other sites More sharing options...
exlimey Posted April 14, 2017 Share Posted April 14, 2017 On 4/10/2017 at 11:03 AM, SMILLER said: In general, we do not use potentiators if we go to a prewarm. Scott 9 hours ago, Sherie Bruns said: We do not use potentiators during a prewarmm. Presumably the operators check that the troublesome reactivity that they are trying to circumvent is actually present in the unpotentiated test ? Ensis01 1 Link to comment Share on other sites More sharing options...
Eagle Eye Posted April 23, 2017 Share Posted April 23, 2017 we do not use potentiator with prewarm... ANORRIS 1 Link to comment Share on other sites More sharing options...
tkakin Posted April 24, 2017 Share Posted April 24, 2017 We do use potentiator with the prewarm. We also use it when testing our WAHA patients. I know there are many labs who do not use it for WAHA workups. I was told that in the end the potentiator really only reduced your incubation time. If this is incorrect someone please enlighten me Link to comment Share on other sites More sharing options...
ANORRIS Posted April 25, 2017 Share Posted April 25, 2017 We do not use potentiator with a prewarm. We incubate for 15 separate then 30 together. Yanxia 1 Link to comment Share on other sites More sharing options...
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