spavlis Posted March 13, 2017 Share Posted March 13, 2017 Does anyone know where we can buy the glycerol solutions used for the blood refrigerators?? The glycerol solution is the solution the sensors and thermometers are placed in the blood refrigerator. Thanks Stephanie Link to comment Share on other sites More sharing options...
Dansket Posted March 13, 2017 Share Posted March 13, 2017 Try www.helmerinc.com. They have solutions for both refrigerator and freezer. Link to comment Share on other sites More sharing options...
Johnv Posted March 14, 2017 Share Posted March 14, 2017 Mallinckrodt manufactures Glycerol USP. I think we ordered directly from the company a couple years ago but I would guess one of the lab supply companies can get it too. Link to comment Share on other sites More sharing options...
SMILLER Posted March 14, 2017 Share Posted March 14, 2017 We just buy glycerol and make up our own 10% solution. Scott Eagle Eye, tricore and TreeMoss 3 Link to comment Share on other sites More sharing options...
carolyn swickard Posted March 14, 2017 Share Posted March 14, 2017 Believe it or not - if you are in a hospital - check with your own pharmacy. Ours stocked glycerol in the pharmacy for use on dry skin. BldBnker and AMcCord 2 Link to comment Share on other sites More sharing options...
exlimey Posted March 15, 2017 Share Posted March 15, 2017 Just curious....why glycerol ? Does the refrigerator manufacturer require you to use it ? Link to comment Share on other sites More sharing options...
spavlis Posted March 15, 2017 Author Share Posted March 15, 2017 22 minutes ago, exlimey said: Just curious....why glycerol ? Does the refrigerator manufacturer require you to use it ? I believe.....Thermometers are placed in solutions similar to the components of blood. 10% glycerol solutions is determined to be similar to blood. BldBnker 1 Link to comment Share on other sites More sharing options...
spavlis Posted March 15, 2017 Author Share Posted March 15, 2017 On 3/14/2017 at 9:28 AM, SMILLER said: We just buy glycerol and make up our own 10% solution. Scott What is formula (recipe??) for making this up?? Link to comment Share on other sites More sharing options...
Dan87 Posted March 15, 2017 Share Posted March 15, 2017 1 hour ago, spavlis said: What is formula (recipe??) for making this up?? C1 x V1 = C2 x V2 Link to comment Share on other sites More sharing options...
jsylvain25 Posted March 15, 2017 Share Posted March 15, 2017 we order from Sigma-Aldrich glycerol for molecular biology >99% it's much cheaper than ordering from helmer Eagle Eye 1 Link to comment Share on other sites More sharing options...
spavlis Posted March 15, 2017 Author Share Posted March 15, 2017 24 minutes ago, jsylvain25 said: we order from Sigma-Aldrich glycerol for molecular biology >99% it's much cheaper than ordering from helmer Do you directly add it or is further dilution needed?? Link to comment Share on other sites More sharing options...
exlimey Posted March 16, 2017 Share Posted March 16, 2017 20 hours ago, spavlis said: I believe.....Thermometers are placed in solutions similar to the components of blood. 10% glycerol solutions is determined to be similar to blood. Is that an official rule, standard, regulation, or something contrived that we've all convinced ourselves is true ? Link to comment Share on other sites More sharing options...
mld123 Posted March 16, 2017 Share Posted March 16, 2017 I just bought Glycerin from Fisher recently and we just make a 10% solution ourselves. Eagle Eye 1 Link to comment Share on other sites More sharing options...
BankerGirl Posted March 16, 2017 Share Posted March 16, 2017 We also bought our glycerin from the local pharmacy. Dilute it to 10% with water. I keep a diluted bottle in the refrigerator for top-offs and the concentrate at room temp. Link to comment Share on other sites More sharing options...
Dan87 Posted March 16, 2017 Share Posted March 16, 2017 2 hours ago, BankerGirl said: We also bought our glycerin from the local pharmacy. Dilute it to 10% with water. I keep a diluted bottle in the refrigerator for top-offs and the concentrate at room temp. BankerGirl, why 10% ? We do 30% and I am wondering if we are over concentrating our glycerol. Link to comment Share on other sites More sharing options...
DPruden Posted March 16, 2017 Share Posted March 16, 2017 4 hours ago, exlimey said: Is that an official rule, standard, regulation, or something contrived that we've all convinced ourselves is true ? It's a CAP checklist item. TRM.42650 Monitored Temperature The temperature of refrigerators is monitored in a manner that will mimic the temperature characteristics of a component stored in the device. NOTE: For example, placement of the temperature sensor probe in liquid with heat transfer characteristics similar to blood, and a volume similar to the smallest units stored, is recommended, but other procedures are also acceptable. The correct placement for the temperature sensor is controversial. Some experts recommend leaving the sensor exposed to air, some recommend enclosing it in liquid, and some recommend enclosing it in an aluminum block. exlimey 1 Link to comment Share on other sites More sharing options...
exlimey Posted March 17, 2017 Share Posted March 17, 2017 18 hours ago, DPruden said: It's a CAP checklist item. TRM.42650 Monitored Temperature The temperature of refrigerators is monitored in a manner that will mimic the temperature characteristics of a component stored in the device. NOTE: For example, placement of the temperature sensor probe in liquid with heat transfer characteristics similar to blood, and a volume similar to the smallest units stored, is recommended, but other procedures are also acceptable. The correct placement for the temperature sensor is controversial. Some experts recommend leaving the sensor exposed to air, some recommend enclosing it in liquid, and some recommend enclosing it in an aluminum block. Perfect ! Thanks for the reference. So...a follow-on question: Who determined that 10% glycerol is a "liquid with heat transfer characteristics similar to blood" ? At least one user on this site is using 30% glycerol. Link to comment Share on other sites More sharing options...
SMILLER Posted March 17, 2017 Share Posted March 17, 2017 The 10% solution (we use a simple 1:9 v/v dilution) has been what has been used traditionally and seems to be one of those things that inspectors don't get too excited about. With glycerol being a bit more dense than water this would be more like a 13% w/w solution. (I believe 10% w/w is close to the thermal properties of plasma and 30% closer to packed RBCs.) Scott Eagle Eye and exlimey 2 Link to comment Share on other sites More sharing options...
BankerGirl Posted March 17, 2017 Share Posted March 17, 2017 On 3/16/2017 at 9:52 AM, Dan87 said: BankerGirl, why 10% ? We do 30% and I am wondering if we are over concentrating our glycerol. 4 hours ago, exlimey said: Perfect ! Thanks for the reference. So...a follow-on question: Who determined that 10% glycerol is a "liquid with heat transfer characteristics similar to blood" ? At least one user on this site is using 30% glycerol. To answer Dan87, we use 10% because that's what we always have used. Not a good reason, I know, which is why I googled this after reading your question. I found one post that states the heat transfer characteristics of components is different, like Scott said. Then I dug further and found a statement in "Textbook of Blood Banking and Transfusion Medicine" that said water is acceptable because it is less dense than the blood components and would, therefore, fluctuate faster than the blood components and allow action to be taken before said components would actually be affected. SO, I think we will stick with 10% since mostly what we have is packed red cells. SMILLER and exlimey 2 Link to comment Share on other sites More sharing options...
TRabs10 Posted October 11, 2017 Share Posted October 11, 2017 Is a 10% glycerol solution OK for a -70 C freezer, or do I need a higher concentration of glycerol? Or, do I need something else all together? Thanks! Link to comment Share on other sites More sharing options...
SMILLER Posted October 11, 2017 Share Posted October 11, 2017 I am not sure, but I would think a glycerol solution might freeze solid at -70. We use a granular material for those ultra-low freezers. It comes in a vial that I believe we get from Cardinal Health. Scott Link to comment Share on other sites More sharing options...
Eagle Eye Posted October 15, 2017 Share Posted October 15, 2017 Yes. 10% glycerol will freeze. We use 50% propylene glycol for freezer. exlimey and mollyredone 2 Link to comment Share on other sites More sharing options...
mollyredone Posted October 16, 2017 Share Posted October 16, 2017 20 hours ago, Eagle Eye said: Yes. 10% glycerol will freeze. We use 50% propylene glycol for freezer. That's what we do as well. Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now