jollymon Posted February 1, 2017 Share Posted February 1, 2017 When testing DTT-treated K+ and K- cells for QC, should you use AHG anti-K, monoclonal anti-K or does it matter? Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted February 2, 2017 Share Posted February 2, 2017 I don't know for certain, but the point is that dithiothreitol is a reducing agent that will break disulphide bonds. The Kell glycoprotein is highly folded due to many disulphide bonds from the 15 cysteine residues in the molecule. It is the breaking of these disulphide bonds that prevents expression of the Kell Blood Group System antigens, as the glycoprotein loses its tertiary structure. It would seem logical, therefore, that either AHG anti-K or Monoclonal anti-K could be used, but, I stress again, I am not certain. exlimey 1 Link to comment Share on other sites More sharing options...
David Saikin Posted February 2, 2017 Share Posted February 2, 2017 11 hours ago, jollymon said: When testing DTT-treated K+ and K- cells for QC, should you use AHG anti-K, monoclonal anti-K or does it matter? Why would you treat/test K= rbcs? You want to see if the K ag is being destroyed so your untreated K+ cell is your positive ct, the treated cell should be negative. exlimey and Malcolm Needs 2 Link to comment Share on other sites More sharing options...
Ensis01 Posted February 8, 2017 Share Posted February 8, 2017 Test a K+ or k+ DTT treated cell by your normal method, if you have a choice use the quickest method. exlimey 1 Link to comment Share on other sites More sharing options...
jollymon Posted February 9, 2017 Author Share Posted February 9, 2017 Thank you all for your replies!! Very helpful! Malcolm Needs 1 Link to comment Share on other sites More sharing options...
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