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heathervaught

Positive Control for DAT on Vision

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We are validating DAT on the Ortho Vision platform, and they do not provide a positive control reagent for this purpose.  What are other Vision users using for positive control for DAT on this platform?

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If you are using the ALBAQ controls we use sample 4 and spin it down and remove the supernatant (or most of it) and add 4 or 5 drops of anti-D to it then re-centrifuge. This will give you a positive result for controls. I am currently in the process of validating the Vision and performing correlations, but this is what we use for QC on the ProVue. 

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We make our positive and negative DAT controls using discarded unit segments. Place the Rh negative blood from 4 to 5 segments in a 12 x 75 glass tube labeled DAT negative and blood from the Rh positive units, again 4 to 5 segments is enough, with 2 drops of anti-D in a tube labeled DAT positive. Invert the tube with the anti-D to mix. Centrifuge the tubes and place on the analyzer. Use the Assign to Position Icon to identify the DAT samples, then choose Apply to Sample.  We registered our DAT control as dat pos and dat neg, so they are recognized when we assign the position.These DAT control last about two weeks for us and work just great (and probably would last longer). We keep a log of the unit numbers used in the preparation of the samples and the unit expiration dates.

 

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2 hours ago, Gkloc said:

If you are using the ALBAQ controls we use sample 4 and spin it down and remove the supernatant (or most of it) and add 4 or 5 drops of anti-D to it then re-centrifuge. This will give you a positive result for controls. I am currently in the process of validating the Vision and performing correlations, but this is what we use for QC on the ProVue. 

This is also what we did to QC our ProVue.

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I do like the method purposed using the ALBAQ control # 4 as the DAT positive. We had been making our own as described but this is definitely a better way.  Thanks so much!

 

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4 hours ago, Gkloc said:

If you are using the ALBAQ controls we use sample 4 and spin it down and remove the supernatant (or most of it) and add 4 or 5 drops of anti-D to it then re-centrifuge. This will give you a positive result for controls. I am currently in the process of validating the Vision and performing correlations, but this is what we use for QC on the ProVue. 

We mix Alba-QCheck vial #4 thoroughly and add 40uL of ORTHO Bio-Clone Anti-D.  Mix thoroughly and centrifuge.  This will give a positive DAT and a positive Rh control on ProVue.  We use it for 7 days.

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We also prepare in-house pos DAT control - this is from our procedure (we use AlbaQ vial 4 for the neg DAT control):

  1. Preparation of Pos DAT QC Sample:

    1. Select an Rh Positive red cell unit with a good outdate from the available inventory.

    2. Complete a label with the lot “DPMMYY” (using MMYY as the month and year of preparation; i.e. “DP0516”).  Add the expiration date equal to the expiration date of the red cell unit as well as the date prepared and your initials.  Place a barcoded P10000 LIS accession label on the vial so it can be scanned onto the Vision.

    3. Connect a syringe set to the red cell using the Sterile Connection Device and remove approx. 5mL of packed cells.  Place the red cells into a plastic 12x75mm test tube labeled with the label made above.

    4. Add 2 drops of Anti-D (Ortho Bioclone) to the packed cells, mix well, and incubate for 30 minutes at 37°C.  Mix again about half-way through incubation.

    5. The in-house reagent is now ready for QC testing.  Validation has been performed and shown that this control reacts 1-3+ in the Poly IgG,C3 Card and is stable until the expiration of the red cell unit.

    6. Print the BIQ unit history for the unit used for Pos DAT QC preparation.  Add the lot and expiration assigned to the QC sample, the date and time of preparation, and your initials on the print out.  Submit print out to the Specialist II.

Stephanie

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