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Continuing Issues with Ortho Gel Reagents and or Equipment


SEBaker

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We have an Ortho Vision and we're seeing "?" on virtually all of our antibody screens. The Vision's camera shows some cells hanging just above the cell button. If you look at them manually we wouldn't have even questioned them as being positive. I have ran 4 different lot numbers of IgG cards during the last 2 weeks and see no difference in any of them.

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  • 2 weeks later...

We are still having problems.  We are on our 4th lot of gel cards and screening cells.  These faint, weak positives are happening with panel cells as well as BioRad 3% cells diluted to 0.8%. What are you all doing?  I don't want to revert back to tube but when we've tried every lot we have here - then what?

I can't even get through to technical service this morning :(

Edited by Cathy
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