Jives Posted November 8, 2016 Share Posted November 8, 2016 just had a specimen on which I could not get a front type due to agglutination (1-2+)....I washed the cells to no avail, even tried washing with warm saline...still pos, tried different tubes drawn at different times (all EDTA) and had the same results on all, the backtype was "A" and showed no signs of a cold antibody. I was perplexed, when one of my co-workers told me to try the blue top or clot tube (they had not drawn a clot) low and behold the blue top suspension gave me negative results with anti-B and anti-D (pt was previous A-)!!! has anyone ever seen something like this before?? my co-worker thought maybe a "heterophile antibody" was interfering, but why only with EDTA?? Link to comment Share on other sites More sharing options...
galvania Posted November 10, 2016 Share Posted November 10, 2016 Did you try with a new EDTA tube. It MIGHT be an anti-EDTA - but it might be that there was a problem with that specific tube Link to comment Share on other sites More sharing options...
Jives Posted November 10, 2016 Author Share Posted November 10, 2016 yes, I tried two other EDTA specimen drawn at different times and they were all positive with anti-B and Anti-D Link to comment Share on other sites More sharing options...
Mabel Adams Posted November 12, 2016 Share Posted November 12, 2016 Did the cells autoagglutinate in saline or only if you added reagent? Link to comment Share on other sites More sharing options...
Recommended Posts
Create an account or sign in to comment
You need to be a member in order to leave a comment
Create an account
Sign up for a new account in our community. It's easy!
Register a new accountSign in
Already have an account? Sign in here.
Sign In Now