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Anyone validate prewarm xm using gel?


BBKT

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Ortho says it is OK as long as you perform you own validation, however, they are not willing to give any recommendations. We rarely do prewarm techniques, and our reference lab recommended prewarm for a patient with a strong cold autoantibody.  I would like to use gel instead of tube testing if possible.  Granted, it would be hard to come up with enough patients with cold autoantibodies for a validation study.  Any thoughts or experiences anyone would like to share?

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This is one technique that I would not want to validate in gel.   Gel will enhance reactions and will pick up strong, direct agglutinins.  A microscopic reaction (negative) in tube could be a 1-2+ (positive) in gel.   I don't want to take the chance of enhancing unwanted reactions and would opt tube testing in this case.  I am interested if others have done this and their success or failure.  

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We attempted to validate a "prewarm gel" procedure, but did not have much luck.  As you say, it is not truly a prewarm method due to the 10 min centrifugation at room temp.  It seemed to work for patients with weaker colds, but if the cold ab was strong, the prewarm gel would usually still be positive.  We dropped it.

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