We currently have a 50 year old male in house that had an accident that damaged his foot 3 weeks ago. He arrived septic and has had to have an amputation.
His ABO/Rh gives a B pos with a 4+ anti-D. His gel screen and panel give 1+ results that match up with an anti-D (all others rules out). His autocontrol was positive at 1+ by IgG, neg for compliment. The eluate results matched the original antibody ID. Presently this patient's specimen is on its way to our reference lab. Previous history at another facility lists him as B Pos, screen negative. As far as we know, he has never been transfused.
What are the possibilities (for what appears to be an D auto antibody), and how should he be treated?
Silly question...,but I'd really like to know,what is the difference in choosing between human and monoclonal reagents for ABO/D testing? Is it a first choice from these two?Is it the price?
I work with Bio-Rad reagents and I know they have cards with human reagents but also identical cards with monoclonal reagents.Our lab use only monoclonal ones.
By Jennifer G
Has anyone seen an Anti-D go from negative to 2+ positive to negative? We had negative antibody screens on an elderly A Negative woman from 2012 through 2018. 8 RBCs were transfused during this time. In August 2018, the antibody screen was 2+ positive, Anti-D was identified, and she received 1 RBC. In April 2019, the antibody screen was negative on 2 different occasions. The possibility of the August 2018 specimen being the wrong patient seems unlikely since we use hand-labeled separately armbanded specimens. However, I have never seen a true Anti-D behave this way.
I was wondering if antibody titre is performed on a pregnant mother who previously had HDFN. According to the books, it mentions 'After the first affected pregnancy, the antibody titer is no longer useful'. Therefore does it mean that it doesn't matter what the antibody titre level is, and should be referred to fetal medicine specialist regardless? Or if there is more to this, I would be grateful for some enlightenment