gilmanch Posted June 3, 2015 Share Posted June 3, 2015 Hi everyone, I am working on consistancy on manual differentials, especially on the bands. Looking for advice, education options, guidelines, pictures that have the cell labeled, any procedures. Thanks! Link to comment Share on other sites More sharing options...
SMILLER Posted June 3, 2015 Share Posted June 3, 2015 Unless it has changed recently, I believe the general consensus on band counts is that they provide almost no clinical information that is not already available with the rest of the CBC/Diff and morphology review. We don't worry too much about them here. Having said that, I would think you could just pick any textbook or internet source for the definition of a band and have everyone use that. The most common definition that I have seen has to do with the narrowist part of the nucleus. If it is greater than half of the long diameter of the nucleus, it is probably a metamyelocyte. If that part is so narrow that no chromitin/parachromitin pattern can be discerned, it's a PMN. If it's in between those two, then you have a band. Scott Link to comment Share on other sites More sharing options...
Auntie-D Posted June 3, 2015 Share Posted June 3, 2015 There are only two occasions that band forms are of any significance are in men who have Barr Bodies visible - 1) it indicates the possibility of the person being 47XXY (or 47XXX if female - two Barr bodies will be present), or 2) in the case of a myeloma that is due to an X-related chomosomal translocation. I always check out the Barr Bodies in left shift if the neuts are raised. Link to comment Share on other sites More sharing options...
Alan Neal Posted June 5, 2015 Share Posted June 5, 2015 WBC Differential /Band cell counts:Suggest review these publications:CAP survey 1995 Lab Hematol 1,89 - Inter and Intra observer consistencyEur J Haematol 2006:76: 251-254. Good article Re variation of WBC Diff with a number of morphologist review digital imagesISLH recent publication June 2015: ICSH recommendations for the standardization of nomenclature and grading of peripheral blood cell morphological features - which recommends that band cells be counted with neutrophils (Also directs to an image library)I would question the value of this enumeration in the 21st cent., also consider the significant variation (LoU) of band cell counting..Our local evaluation with digital images, gave a LoU of > 40% (Which is a good result!), hence we do not report bands cell counts, but will only make a comment. kackieanne 1 Link to comment Share on other sites More sharing options...
gilmanch Posted June 12, 2015 Author Share Posted June 12, 2015 Thank you for the input and the article links! Those are very helpful reads!!! Link to comment Share on other sites More sharing options...
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