apfelblosm Posted October 13, 2014 Share Posted October 13, 2014 Hey all! For those that perform the KB stain using the Sure-Tech Kit, I have a few questions for you. We do a ton (10-20) of these a month. We are having problems with our controls staining appropriately. It is hard to determine the positives. Here are the variables we have already examined:1. Happens to variety of techs including seasoned and new techs2. We only use the stain for 5 tests and then we discard it.3. We run high, low and neg controls every time we do the stain.4. We use the same area of the lab every time to try to control temperature. Is anyone else having or has had a problem with this? Thanks!Jen Link to comment Share on other sites More sharing options...
Auntie-D Posted October 13, 2014 Share Posted October 13, 2014 We've been having this issue too and have had to cut staining time to 1 minute for the pink. This still gives the odd bad one The only way round it seems to make controls at the same time as the samples being stained. Link to comment Share on other sites More sharing options...
3cardmoe Posted October 14, 2014 Share Posted October 14, 2014 We use Sure-Tech and haven't had any issues. We store the reagents in a controlled environment of about 23C. And we stain for 3 minutes. We also make controls each time we run patient samples. Link to comment Share on other sites More sharing options...
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