Anti-Jsa to screen or not to screen

[kmmoton]

I would like to know what's everyone's take on a patient with an Anti-Jsa. Do you have units screen or do you just XM Extend?  Please explain your answer.

[galvania]

Dear kmmoton

You would be EXTREMELY lucky to find anti-Jsa to carry out screening tests with!  You might be lucky enough to find some genotyped blood.  Cross-match should be fine, as long as the anti-Jsa is still detectable

[AMcCord]

If this was a patient I would expect to see in the future  for transfusion and his/her antibody was fairly strong, I would freeze the patient's plasma in aliquots to use for prescreening donors prior to crossmatching with a current specimen. This is something that was recommended to us by our reference lab in cases where commercial antisera is not available. If the patient's titer drops later, you will have a little double check for compatibility. If possible, the frozen plasma should be QC'd with an antigen positive cell (from an ID panel) prior to use to see if it is still viable. Of course, that is dependant on what the antibody is/how rare that antigen positive cell is. 

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Edited June 3, 2015 by RollSlow10

[Yanxia]

What is the meaning of AA?

[Malcolm Needs ☆]

African American shily.

 

In that case RollSlow10, I would echo the advice of AMcCord.

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African American shily.

 

In that case RollSlow10, I would echo the advice of AMcCord.

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Edited June 3, 2015 by RollSlow10

[goodchild]

We use cells with heterozygous expression (whenever possible) for positive controls.

[Malcolm Needs ☆]

From what you say above RollSlow10, I would be inclined to adsorb out the anti-C, anti-E, anti-N, anti-K, anti-Fy^a, anti-Jk^b and just leave the anti-Js^a.  This should be easy, as you can use red cells from White donors, who are less likely (by far) to express the Js^a antigen.

 

As a control, you MUST use Js(a+b+) red cells, rather than the incredibly rare Js(a+b-) red cells, but, initially, I would test the adsorbed plasma with both (if available).