yaya Posted November 25, 2013 Share Posted November 25, 2013 for how long we can keep the EDTA specimen at room temperature before start performing the DCT?and if we store it at 2-8 c ,is that going to affect the result !! Link to comment Share on other sites More sharing options...
yaya Posted November 27, 2013 Author Share Posted November 27, 2013 i now it is silly question but any help please Link to comment Share on other sites More sharing options...
galvania Posted November 27, 2013 Share Posted November 27, 2013 No - it's not a silly question.As for most tests, the fresher the sample, the more reliable the result will be. As cells get old, you can get all sorts of non-specific reactions taking place which are more or less visible depending on the method you use. Having said that, I guess you're talking hours rather than days - so there should not be any problem at all.As for storing them in the fridge, as long as it's EDTA that's not a problem as long as you make really sure that they are at room temperature before you use them, otherwise you're going to pick up all sort of cold auto-antibodies that are totally irrelevant. For non-EDTA samples, this is a bit of a no-no, becuase you will get in-vitro complement binding. Link to comment Share on other sites More sharing options...
yaya Posted November 28, 2013 Author Share Posted November 28, 2013 thanks for your replyI was wondering since there is a possibility of detaching the antibodies at the stage of washing before adding AHG -so keeping the cells at room temperature or cold temp will not affect the IgG bounding antibodies i.e. giving negative results or weaker results. Link to comment Share on other sites More sharing options...
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