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MTS Diluent

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What do you use as a positive and negative control to QC the MTS Diluent ?

We use a donor segment O pos as our cells

 

Our serum is

1.  Negative is 6% alb

2.  Positive is diluted anti-D

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We use plasma from a patient of known type who has a negative screen.  Then we crossmatch this "recipient" with donors whose ABO types  will be compatible and incompatible.  Ironic since the gel is not licensed to detect ABO incompatibilities but it has never failed in 10 years! :lol:

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we make up positive DAT specimen by adding anti-D to O Pos donor. We run Positive & Negative(O Neg) DAT on ProVue and by manual Gel. We run it everyday.

Frequency should be decided based on usage pattern and staff availability. We almost always use MTS 2 everyday so we run controls on first shift. Unless there is a big saving I would discourage using day of use as you can not expect midnight or evening(usually short staffed everywhere) shift to find/to make up controls on the day of use.

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Do you perform this daily or only on day of use?

 

We do ours daily since most days we need to due a full gel crossmatch.

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What do you use as a positive and negative control to QC the MTS Diluent ?

There are two diluents, MTS Diluent 2 and MTS Diluent 2PLus that should not cause or prevent agglutination.  If you are doing QC to determine that the diluents are inert in all situations, you would have prepare test cells suspended in each diluent. 

 

You would then have to test the cells in MTS Diluent 2Plus against each gel reagent (anti-A gel, anti-B gel, anti-D gel, Buffered Gel (immed-spin xmatch), Monoclonal Gel) and test cells in MTS Diluent 2 against anti-IgG gel (both DATand -IgG xmatch) using a test cell that gives a positve result and also a test cell that give a negative result.

Edited by Dansket

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We make suspension of the control we make which is Positive DAT(O pos donor with anti-D added to it) & negative DAT(O neg donor). We make suspension with MTS diluent 2 & MTS Diluent 2+ and run it on IgG card.

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19 hours ago, amym1586 said:

What's the Expiration date of MTS Dil 2 after opening?

 

And do you document the cleaning of the pump?  or how often?

From the pkg insert:

Store at 2-8°C.  (no change of expiration once opened)

QC: document visual inspection daily, test with known pos and negative daily

Maintenance of pump: document weekly cleaning with 70% isopropyl alcohol

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On 7/22/2013 at 6:14 AM, LCoronado said:

We use plasma from a patient of known type who has a negative screen.  Then we crossmatch this "recipient" with donors whose ABO types  will be compatible and incompatible.  Ironic since the gel is not licensed to detect ABO incompatibilities but it has never failed in 10 years! :lol:

We were doing some validation recently and found that a B patient (with a weaker than average reverse type) crossmatched to 3 A units in the MTS IgG card failed to detect incompatibility in one of the 3 units.  Of course, tube testing and Grifols gel also failed to detect this incompatibility.  Turns out that the unit was an A2.  As I recall, the various immediate spin methods also were negative with this unit (MTS buffered gel, Grifols neutral gel and tube IS).  This is why computer algorithms are better at picking up ABO incompatibilities than any serological testing.  The computer can't have either false positive or false negatives (as long as we get the types right).

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Totally agree.

We encountered same with ProVue....we tried running IS on buffered gel card and did not detect ABO incompatibility on one occasion!!

Try fighting with regulating bodies .....CLIA interpretation with Ortho letter......Most sites gets cited if you are not shaking tubes for IS when using gel methods......If your computer system is able to detect ABO incompatibility at the unit selection, is far better than doing IS by tube with Gel crossmatch.................but no one wants to listen and the reason being when people get cited they blindly do IS instead of justifying why computer is better than shaking tube for IS......

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