Kathyang Posted April 17, 2013 Share Posted April 17, 2013 We are starting with The Joint Commission inspection in October and noticed they wanted saline QC.I would like to know what anyone is doing for saline QC. Thanks, Kathy Link to comment Share on other sites More sharing options...
David Saikin Posted April 17, 2013 Share Posted April 17, 2013 I do not perform saline qc (but I do check my gel cell diluent daily) - I would check my saline if I discovered unanticipated results when it was used . . . You could use it as a negative "serum" and run with ab screening cells. You could evaluate its appearance (should be clear). Link to comment Share on other sites More sharing options...
Changezi Posted April 17, 2013 Share Posted April 17, 2013 well we are now reciving comercially prepared saline , but for manual i just check with RBC make the suspension centrifuge and check for the hemolysis ,(just forget about my english always for my all post :juggle: Link to comment Share on other sites More sharing options...
Mabel Adams Posted April 18, 2013 Share Posted April 18, 2013 Riaz Ahmed, your English is fine. I am always humbled by all of you that communicate here in a language not your native tongue and I never mind asking for clarification if I don't understand something. I hope you always ask for clarification when needed also. Link to comment Share on other sites More sharing options...
BBCLS Posted April 18, 2013 Share Posted April 18, 2013 We are starting with The Joint Commission inspection in October and noticed they wanted saline QC.I would like to know what anyone is doing for saline QC. Thanks, KathyWe QC all our saline cubes from the washer and squirt bottles every morning. 2 drops of saline 1 drop of pool cells 2 drops of peg Incubate at 37 Wash x4 Add control cells- - - Updated - - -We are starting with The Joint Commission inspection in October and noticed they wanted saline QC.I would like to know what anyone is doing for saline QC. Thanks, KathyWe QC all our saline cubes from the washer and squirt bottles every morning. 2 drops of saline 1 drop of pool cells 2 drops of peg Incubate at 37 Wash x4 Add control cells Link to comment Share on other sites More sharing options...
BBCLS Posted April 18, 2013 Share Posted April 18, 2013 We QC all our saline cubes from the washer and squirt bottles every morning. 2 drops of saline 1 drop of pool cells 2 drops of peg Incubate at 37 Wash x4 Add control cells- - - Updated - - -We QC all our saline cubes from the washer and squirt bottles every morning. 2 drops of saline 1 drop of pool cells 2 drops of peg Incubate at 37 for min. of 10 mins. Wash x4 Add control cellsSorry ! Incomplete. Add IGG , spin , read before your control cells. Link to comment Share on other sites More sharing options...
Emwilson7 Posted April 18, 2013 Share Posted April 18, 2013 We do almost the same as BBCLS, except we don't add enhancement. It's done every morning with daily QC. Link to comment Share on other sites More sharing options...
Mabel Adams Posted April 19, 2013 Share Posted April 19, 2013 I don't remember seeing a JC std that requires this. Can you tell me which standard? Link to comment Share on other sites More sharing options...
goodchild Posted April 19, 2013 Share Posted April 19, 2013 I'm not familiar with the laboratory accreditation requirements for TJC but I assume there is something about following manufacturer's written instructions. Our blood bank saline comes with the instructions: "A sample of this saline should be inspected visually and also tested as a negative control in the indirect antiglobulin test on a daily basis." Link to comment Share on other sites More sharing options...
James Smith Posted April 19, 2013 Share Posted April 19, 2013 Our lab uses buffered saline (pH 7.0-7.2) and the blood gas equipment to test the pH daily. In addition we run a visual inspection of saline and monoclonal diluent control and anti-D with Rh+ and Rh- cells in our daily QC which we run thru the cell washer to see if we get the expected positive or negative reaction agglutination pattern at IAT. Bench saline bottles use same source but not testing every bottle, just the saline 10L cube connected to a Helmer cell washer daily. I haven't seen any problems with saline bottles being contaminated. Link to comment Share on other sites More sharing options...
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