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We are starting with The Joint Commission inspection in October and noticed they wanted saline QC.

I would like to know what anyone is doing for saline QC.

Thanks,

Kathy

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I do not perform saline qc (but I do check my gel cell diluent daily) - I would check my saline if I discovered unanticipated results when it was used . . . You could use it as a negative "serum" and run with ab screening cells. You could evaluate its appearance (should be clear).

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well we are now reciving comercially prepared saline , but for manual i just check with RBC make the suspension centrifuge and check for the hemolysis ,

(just forget about my english always for my all post :juggle:

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Riaz Ahmed, your English is fine. I am always humbled by all of you that communicate here in a language not your native tongue and I never mind asking for clarification if I don't understand something. I hope you always ask for clarification when needed also.

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We are starting with The Joint Commission inspection in October and noticed they wanted saline QC.

I would like to know what anyone is doing for saline QC.

Thanks,

Kathy

We QC all our saline cubes from the washer and squirt bottles every morning.

2 drops of saline

1 drop of pool cells

2 drops of peg

Incubate at 37

Wash x4

Add control cells

- - - Updated - - -

We are starting with The Joint Commission inspection in October and noticed they wanted saline QC.

I would like to know what anyone is doing for saline QC.

Thanks,

Kathy

We QC all our saline cubes from the washer and squirt bottles every morning.

2 drops of saline

1 drop of pool cells

2 drops of peg

Incubate at 37

Wash x4

Add control cells

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We QC all our saline cubes from the washer and squirt bottles every morning.

2 drops of saline

1 drop of pool cells

2 drops of peg

Incubate at 37

Wash x4

Add control cells

- - - Updated - - -

We QC all our saline cubes from the washer and squirt bottles every morning.

2 drops of saline

1 drop of pool cells

2 drops of peg

Incubate at 37 for min. of 10 mins.

Wash x4

Add control cells

Sorry ! Incomplete. Add IGG , spin , read before your control cells.

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I'm not familiar with the laboratory accreditation requirements for TJC but I assume there is something about following manufacturer's written instructions. Our blood bank saline comes with the instructions: "A sample of this saline should be inspected visually and also tested as a negative control in the indirect antiglobulin test on a daily basis."

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Our lab uses buffered saline (pH 7.0-7.2) and the blood gas equipment to test the pH daily. In addition we run a visual inspection of saline and monoclonal diluent control and anti-D with Rh+ and Rh- cells in our daily QC which we run thru the cell washer to see if we get the expected positive or negative reaction agglutination pattern at IAT. Bench saline bottles use same source but not testing every bottle, just the saline 10L cube connected to a Helmer cell washer daily. I haven't seen any problems with saline bottles being contaminated.

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