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Searching for LN2 Autofilling tank


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To freeze cells and store cells usually requires different equipment. I only know about freezing stem cells, which requires a controlled rate freezer that drops the temp gradually to -80, at which point the cells are transferred into the long term storage freezer at -190 ish.

For controlled rate freezing we use a Cryomed model 1010 (ancient) purchased from Custom Biogenics systems http://www.custombiogenics.com/ I dont know whether this would be appropriate for freezing rbcs, Im sure a blood supplier reader is out there and will chime in :)

For long term LN2 storage, we use the Chart MVE 819 with TEC 3000 controller http://www.northeastcryo.com/pdfs/10934034.pdf

Dont know where you are located but we purchased ours from northeast services in south plainfield NJ, which also provides service (great company) http://www.northeastcryo.com/ It holds a very stable temp for a long time (approx 2 days between fills) on a full charge. We run with btw 4-6 inches LN2 which keeps temps around -190 continuously. The controller unit tracks LN2 levels, and when you drop below whatever level youve set it opens the fill valve automatically, until your target level is reached, at which point it shuts the valve. All you need is an external supply tank, although it is possible to run a permanent piped system for supply. Which model you buy is going to depend on the number of products you anticipate storing. Most companies that sell these support a variety of racking systems and which one you choose will affect the total number of bags that can be stored. Id strongly suggest something with built in temp/Ln2 level monitoring and alarms that can be wired to a remote monitoring system. We needed an external chart recorder to maintain a record of our continuous temps since our Isensix monitoring system does not have its own temp probe for LN2, it only says yes/no you are in alarm state. Other monitoring systems may offer this capability.

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