melvolny Posted August 8, 2012 Share Posted August 8, 2012 We have an OB patient with an anti-E. This morning when I titered it, it gave me some very odd reactions, something I have never seen an antibody do before. The repeat was the same. I performed the titer in gel.The first (raw) tube was weak, the second tube was 1+ and the third tube was a strong 2+, the rest were negative.I repeated it thinking I set up the tubes incorrectly. The repeat was the exact same with tube 4 being weakly positive. The rest were negative.Anybody else ever see anything like this? And what's up with the antibody that would cause this?Thanks in advance for any and all responses.Melissa Link to comment Share on other sites More sharing options...
Mabel Adams Posted August 9, 2012 Share Posted August 9, 2012 You used to hear about prozone but I don't know if you see it in gel and I think the titer would be much higher than that. Link to comment Share on other sites More sharing options...
galvania Posted August 9, 2012 Share Posted August 9, 2012 You CAN see prozone in gel - but yes, usually it is with stronger antibodies. I have never seen one before at this titre (DiaMed gel - can't speak for the others) Link to comment Share on other sites More sharing options...
David Saikin Posted August 10, 2012 Share Posted August 10, 2012 Why don't you try your titer in tubes (based on your results I would bet that you find no reactivity). . . were you using Ortho cells? I'd try a different vendor's cells and see what you get. I have had R2R2 Ortho cells react in gel but no other vendor's R2R2 would react unless enzyme pretreated. Link to comment Share on other sites More sharing options...
melvolny Posted August 10, 2012 Author Share Posted August 10, 2012 Thanks for all your replies! Right after I posted this - my brain did the old "Of course - prozone!" I thought I would titer it in the tube next - just to see what it does. I, too, was very surprised it wasn't a higher titer than that. We'll see what happens as the pregnancy progresses.Thanks again! Link to comment Share on other sites More sharing options...
rravkin@aol.com Posted August 12, 2012 Share Posted August 12, 2012 I wonder if this is a new Anti E and the concentrations of IgM and IgG fractions are somehow involved in these reactions. Link to comment Share on other sites More sharing options...
DPruden Posted September 6, 2012 Share Posted September 6, 2012 I wonder if this is a new Anti E and the concentrations of IgM and IgG fractions are somehow involved in these reactions.I was going to suggest this as well, we had a patient with a persistent IgM anti-E that never seroconverted to IgG. Link to comment Share on other sites More sharing options...
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