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Saline incubation...why is this SOP still allowed?


labgirl153

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On 8/20/2012 at 6:37 AM, yan xia said:

Auto-antibody is a very headache question worry me, if saline incubation can resolve it , I will be very happy.

I am very interesting in how to do it, and is it useful when the auto is 37 degree reactive and very strong. Thanks for your opinion.:P

Rarely you will find an WAA stronger than an underlying alloantibody. That is what makes this method useful. The weaker autoantibodies won’t react and only the stronger alloantibodies will react.  Attached is the AABB sop for this method. 

METHOD 3-2. SALINE INDIRECT ANTIGLOBULIN TEST PROCEDURE.pdf

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