Fetal Bleed Confirmatory Testing by Flow Cytometry

[Quality_Lady]

Has any facility implemented the Quotient flow methodology for confirming fetal bleeds? We are looking at using it to do testing and are in the planning stages of how to “verify / validate†the assay. If you did implement, can I ask what you did to implement the assay and verify that it worked in your hands?

Thanks!

[Colin Barber]

Not sure if this will help - this is the SOP for FMH by flow cytometry done for us by our Immunophenotyping lab. We do the traditional Kleihauer in Blood Transfusion and any positives over 2ml of FMH we get confirmed by our colleagues in the flow lab.

Colin

Investigation of FMH by Flow Cytometry.doc

[Quality_Lady]

Not sure if this will help - this is the SOP for FMH by flow cytometry done for us by our Immunophenotyping lab. We do the traditional Kleihauer in Blood Transfusion and any positives over 2ml of FMH we get confirmed by our colleagues in the flow lab.

Colin

Thanks Colin -

This is some good information. Can you provide any clarification as to what you did to initially verify the assay in your lab?

Thanks and Happy New Year!

[Colin Barber]

Thanks Colin -

This is some good information. Can you provide any clarification as to what you did to initially verify the assay in your lab?

Thanks and Happy New Year!

The validation performed by our colleagues in the Immunophenotyping Lab were:

1) To make a series of dilutions of cord cells in group compatible adult blood, to represent FHM of know volume.

2) To run any of our positive Kelihauers in parallel with the RCI Lab in our National Blood Service - this is where we would send samples normally for confirmation of FMH.

3) To test the NEQAS samples sent to us for FMH.

I have also attached a method supplied by our NEQAS lab for making positive samples.

Colin

preparing FMH samples.doc

[Quality_Lady]

Thanks Colin -

This is just the information that I needed.