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Auto PBSC collection


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For those of you collecting HPC's using the Spectra AutoPBSC program, what do you have your harvest and chase volumes set at?

We collect autologous HPC's only. After years of doing collections using the MNC program with operator control we went to the Auto PBSC several months ago. As expected, we saw the counts in our collections decrease. For our last patient we set the harvest volume at 5 ml and the chase volume at 6 ml. That seemed to make an improvemnt. Other than cryopreserving, we do not manipulate the HPC's post collection and again, our collections are autologous only. Does anyone with Auto PBSC experience have suggestions on which direction we should go in, volume wise?

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