Problems with Anti-A reagent

[Beth Eades]

Good afternoon! Has anyone had any reports or experienced false positive results with Biotest Anti-A reagent? Thanks in advance.

Beth Johnson

[Eagle Eye]

did you check the vial for turbidity? Is your negative control Negative?

[Beth Eades]

No turbidity, controls are fine...

[Yanxia]

Does the cells washed before testing? If not , there maybe anti-prenservation reagent antibodies in the plasma give the false postive result.

And if my memeory don't treat me, ther is another posipility is Tn active like A antigen.

[Yanxia]

And antibodies in the anti-A reagent to red cells low frequency antigen.

The first one we can wash the cells to avoid it, the second one we can test the cells with AB serum to detect the secret antigen exposue, in the third one, I think it maybe change to other reagent.

[galvania]

What problems exactly and what type of reagent?

[David Saikin]

Good question Galvania - it is helpful to know what type of problem you are experiencing and also if the reagent is human-based or monoclonal. How do you know that there are false positives - previous patients?

Edited June 4, 2010 by David Saikin
wanted to qualify statement