javvcr Posted July 27, 2009 Share Posted July 27, 2009 i have to ask you if someone have ever been in this situation berofe!in our lab, we used to performe our panel control weekly, using a commercial anti Jka (IAT use).but in this occation, with a new lot, and a new supplier, our Panel (Diamed), give us a nice identification, but our Panel E (papain treated, and Diamed too), give us all the eleven cells, negative. I was so concern but, i decide to use bromeline treated Cell (surse from the untreated panel) and the reaction were enhanced like i spected.Resuming, our Panel E, enhance perfectly, all the others antibodies, but, this one, this particular supplier, didn't. have someone ever have a situation like that before!jimmy Link to comment Share on other sites More sharing options...
galvania Posted August 3, 2009 Share Posted August 3, 2009 It's probably due to the particular antiserum you are using. Commercial antisera are all 'cooked' in some way or other, and don't necessarily behave in the same way as real patient antibodies - especially if the antisera are monoclonal. Link to comment Share on other sites More sharing options...
Malcolm Needs ☆ Posted August 3, 2009 Share Posted August 3, 2009 I would agree with Anna.We sometimes get antibodies in our external QA scheme that react perfectly well in IAT, but do not react as would be expected with enzyme-treated red cells. These antibodies are also "mucked about with".Terrible English I know! Link to comment Share on other sites More sharing options...
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