EluKit last wash

[JOANBALONE]

Does anyone have any information about the protien neutralization test to test the last wash when using EluKit? We may start to do this test but can not find any information about it.

[David Saikin]

The last wash is only wash solution/(LIS) - it does not need to be neutralized. At least per the package insert. I run the last wash with screening cells . . . it should be negative for your eluate to be valid.

[bpkelly]

Send me you e mail and I will send you my procedure

[GilTphoto]

The last wash is only wash solution/(LIS) - it does not need to be neutralized. At least per the package insert. I run the last wash with screening cells . . . it should be negative for your eluate to be valid.

This is what we do. The purpose in testing is to ensure any serum antibody has been removed before adding the eluting solution. If it's positive, continue to wash the cells.

[Philip Thornton]

The last wash is only wash solution/(LIS) - it does not need to be neutralized. At least per the package insert. I run the last wash with screening cells . . . it should be negative for your eluate to be valid.

We do the same; the last wash should be performed with screen cells as a negative control to ensure that the washing was effective.