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HPC Viabilities


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Hi all

We are comparing the pre-processing and post processing (at least 48 hours after step-down freeze) viabilities of our HPC units and finding quite a bit of variation.

Do other HPC processing labs do this kind of comparison? If so, what are the limits of difference for acceptability? I haven't been able to find any benchmarks for this to date.

Any help would be welcome…

thanks

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We have recently began doing post-thaw testing and comparing it to the fresh testing, however, we are performing viable CD34 % recovery post testing. We are using a flow lab that tests for viable CD34 using 7-AAD and single platform methodology so I get comparable absolute CD34 values both fresh and post thaw. We have set a limit of 50% recovery, or we will alert physician of possible poor/sluggish engraftment outcomes. We also calculate the dose of viable CD34 cells/Kg after freezing as well, so the physician has that to look at too. We report the post freeze viability on our reports for information, but have no standard or cut-off limits set. Since this is a viability for all cells in the product (not necessarily CD34 cells), you could get very poor viability just because you have a high granulocyte percentage which died during the freeze but your CD34 cells are fine. That's why we chose to to use CD34 viable recovery.

There are a lot of excellent articles that can be review on this subject on the internet. Dr. Rob Sutherland has put out quite a bit of research on this.

Good luck!

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