dpflanz Posted August 15, 2006 Share Posted August 15, 2006 Hi allWe are comparing the pre-processing and post processing (at least 48 hours after step-down freeze) viabilities of our HPC units and finding quite a bit of variation. Do other HPC processing labs do this kind of comparison? If so, what are the limits of difference for acceptability? I haven't been able to find any benchmarks for this to date.Any help would be welcome…thanks Link to comment Share on other sites More sharing options...
L106 Posted October 23, 2009 Share Posted October 23, 2009 If no BBTalk users respond, do you have other facilities handling HPC units that you can consult? Link to comment Share on other sites More sharing options...
Shadya Posted December 23, 2009 Share Posted December 23, 2009 We have recently began doing post-thaw testing and comparing it to the fresh testing, however, we are performing viable CD34 % recovery post testing. We are using a flow lab that tests for viable CD34 using 7-AAD and single platform methodology so I get comparable absolute CD34 values both fresh and post thaw. We have set a limit of 50% recovery, or we will alert physician of possible poor/sluggish engraftment outcomes. We also calculate the dose of viable CD34 cells/Kg after freezing as well, so the physician has that to look at too. We report the post freeze viability on our reports for information, but have no standard or cut-off limits set. Since this is a viability for all cells in the product (not necessarily CD34 cells), you could get very poor viability just because you have a high granulocyte percentage which died during the freeze but your CD34 cells are fine. That's why we chose to to use CD34 viable recovery. There are a lot of excellent articles that can be review on this subject on the internet. Dr. Rob Sutherland has put out quite a bit of research on this.Good luck! Link to comment Share on other sites More sharing options...
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